Variability of acute extracellular action potential measurements with multisite silicon probes.
Variability of acute extracellular action potential measurements with multisite silicon probes.
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DOI：
10.1016/j.jneumeth.2012.08.005
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发表时间：
2012-10-15
影响因子：
3
通讯作者：
Masmanidis, Sotiris C.
中科院分区：
文献类型：
--
作者：
Scott, Kimberly M.;Du, Jiangang;Lester, Henry A.;Masmanidis, Sotiris C.
设备小型化技术已经导致用于脑电活动的细胞外测量的传感器的显著进步。含有植入式电极阵列的多位点硅基探针比单电极提供更大的神经元活动覆盖范围，因此可能提供神经元集合如何编码信息的更完整视图。然而，按比例增加位点的数量不足以确保捕获多个神经元，因为来自细胞外电极的动作电位信号可能由于许多因素而变化。为了了解多位点探针的大规模记录能力和潜在限制，重要的是量化这种可变性，并确定某些关键设备参数是否影响记录。在这里，我们调查四个参数，即，电极表面，宽度的结构支撑轴，轴数，和位置的记录网站相对于轴尖的影响。本研究采用急性植入硅探针包含多达64个记录站点，其性能进行评估的噪声，尖峰幅度，尖峰检测概率的度量。平均而言，我们发现设备几何形状对尖峰幅度和检测概率没有显着影响，但我们发现个体实验之间存在显着差异，检测尖峰的可能性在试验中变化约为3倍。
Device miniaturization technologies have led to significant advances in sensors for extracellular measurements of electrical activity in the brain. Multisite, silicon-based probes containing implantable electrode arrays afford greater coverage of neuronal activity than single electrodes and therefore potentially offer a more complete view of how neuronal ensembles encode information. However, scaling up the number of sites is not sufficient to ensure capture of multiple neurons, as action potential signals from extracellular electrodes may vary due to numerous factors. In order to understand the large-scale recording capabilities and potential limitations of multisite probes, it is important to quantify this variability, and to determine whether certain key device parameters influence the recordings. Here we investigate the effect of four parameters, namely, electrode surface, width of the structural support shafts, shaft number, and position of the recording site relative to the shaft tip. This study employs acutely implanted silicon probes containing up to 64 recording sites, whose performance is evaluated by the metrics of noise, spike amplitude, and spike detection probability. On average, we find no significant effect of device geometry on spike amplitude and detection probability but we find significant differences among individual experiments, with the likelihood of detecting spikes varying by a factor of approximately three across trials.
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