Plasmonic photoreactors-coated plastic tubing as combined-active-and-passive antimicrobial flow sterilizer.
Plasmonic photoreactors-coated plastic tubing as combined-active-and-passive antimicrobial flow sterilizer.
复制标题
等离子体光反应器涂层塑料管作为组合主动和被动抗菌流灭菌器。
DOI：
10.1039/d1tb02250d
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发表时间：
2022-03-23
影响因子：
7
通讯作者：
Reinhard, Bjorn M.
中科院分区：
文献类型：
--
作者：
An, Xingda;Cheng, Ronghai;Liu, Pinghua;Reinhard, Bjorn M.
塑料材料在医疗器械和消费品中无处不在。由于塑料表面的细菌污染可能造成重大的健康风险，因此需要有效的方法来抑制塑料表面上的细菌并防止塑料表面的定殖。在这项研究中，我们评估了塑料表面的等离子体光反应器涂层，提供主动和被动的抗菌效果，并实现可见光驱动的抗菌流灭菌器。我们证明了这种方法灭活了通过光反应器涂层聚乙烯管的水悬浮液中的细菌，在共振LED照明下，革兰氏阳性和阴性细菌的对数减少值（LRV）> 5。重要的是，抗微生物流动灭菌器不会导致单克隆抗体的可检测功能损失，这些单克隆抗体作为需要灭菌的高价值生物制品的示例纳入本工作。在环境光照射下，等离子体光反应器涂层对细菌定殖和生物膜形成表现出显著的抑制作用。对哺乳动物细胞的抑制作用明显较弱，表明涂层提供的保护具有一定的选择性。
Plastic materials are ubiquitous in medical devices and consumer goods. As bacterial contamination of plastic surfaces can pose significant health risks, there is a need for effective approaches both to inactivate bacteria on plastic surfaces and to prevent colonization of plastic surfaces. In this study, we evaluate a plasmonic photoreactor coating for plastic surfaces that provides both active and passive antimicrobial effects and implement a visible light-driven antibacterial flow sterilizer. We demonstrate that this approach inactivates bacteria in an aqueous suspension passed through a photoreactor-coated polyethylene tubing, achieving log reduction values (LRVs) > 5 for both Gram-positive and -negative bacteria under resonant LED illumination. Importantly, the antimicrobial flow sterilizers do not cause a detectable loss of functionality for monoclonal antibodies that were included in this work as an example of high-value biologics that require sterilization. Under ambient light illumination, the plasmonic photoreactor coating exhibits a significant inhibitory effect on bacterial colonization and biofilm formation. The inhibitory effect was substantially weaker for mammalian cells, indicating some selectivity in the protection provided by the coating.
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影响因子：
6.7
作者：
An X;Erramilli S;Reinhard BM
通讯作者：
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