Fibronectin binding protein B binds to loricrin and promotes corneocyte adhesion by Staphylococcus aureus.
Fibronectin binding protein B binds to loricrin and promotes corneocyte adhesion by Staphylococcus aureus.
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DOI：
10.1038/s41467-022-30271-1
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发表时间：
2022-05-06
影响因子：
16.6
通讯作者：
中科院分区：
文献类型：
--
作者：
金黄色葡萄球菌在人类中的定植是感染的主要风险因素，但所涉及的细菌和宿主因素尚未完全了解。皮肤定殖过程中的第一步是细菌粘附到角质层中的角质细胞，其中角质层包膜蛋白兜甲蛋白是S.金黄色。在这里，我们报告了一个新的兜甲蛋白结合蛋白的S。金黄色葡萄球菌，细胞壁锚定的纤连蛋白结合蛋白B（FnBPB）。单分子力谱显示弱和超强（2 nN）的FnBPB结合兜甲蛋白和机械应力增强这些债券的强度。用来自纤维蛋白原的肽处理降低了强相互作用的频率，表明两种配体结合到FnBPB内的重叠位点。最后，我们表明，FnBPB促进粘附到人角质细胞的结合强烈兜甲蛋白，突出了这种相互作用的相关性，皮肤定植。在皮肤定植过程中，第一步是其粘附到角质细胞。Da Costa等发现，S.金黄色葡萄球菌与兜甲蛋白结合。应用单细胞力谱，他们证明了这种相互作用促进了S。金黄色葡萄球菌对人角质细胞的作用。
Colonisation of humans by Staphylococcus aureus is a major risk factor for infection, yet the bacterial and host factors involved are not fully understood. The first step during skin colonisation is adhesion of the bacteria to corneocytes in the stratum corneum where the cornified envelope protein loricrin is the main ligand for S. aureus. Here we report a novel loricrin-binding protein of S. aureus, the cell wall-anchored fibronectin binding protein B (FnBPB). Single-molecule force spectroscopy revealed both weak and ultra-strong (2 nN) binding of FnBPB to loricrin and that mechanical stress enhanced the strength of these bonds. Treatment with a peptide derived from fibrinogen decreased the frequency of strong interactions, suggesting that both ligands bind to overlapping sites within FnBPB. Finally, we show that FnBPB promotes adhesion to human corneocytes by binding strongly to loricrin, highlighting the relevance of this interaction to skin colonisation. The first step during skin colonization by is its adhesion to corneocytes. Da Costa et al. show that the cell wall-anchored fibronectin binding protein B (FnBPB) of S. aureus binds to loricrin. Applying single cell force spectroscopy, they demonstrate that this interaction promotes adhesion of S. aureus to human corneocytes.
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金黄色葡萄球菌块状因子A是一种激活细菌粘附力的力敏感分子开关。
DOI：
10.1073/pnas.1718104115
发表时间：
2018-05-22
影响因子：
11.1
作者：
Herman-Bausier P;Labate C;Towell AM;Derclaye S;Geoghegan JA;Dufrêne YF
通讯作者：
Dufrêne YF
影响因子：
6.4
作者：
Herman-Bausier P;El-Kirat-Chatel S;Foster TJ;Geoghegan JA;Dufrêne YF
通讯作者：
Dufrêne YF
影响因子：
3.1
作者：
Ahmed, S;Meghji, S;Nair, SP
通讯作者：
Nair, SP
影响因子：
1.6
作者：
HUTTER, JL;BECHHOEFER, J
通讯作者：
BECHHOEFER, J
影响因子：
17.1
作者：
Alsteens, David;Dupres, Vincent;Klotz, Stephen A.;Gaur, Nand K.;Lipke, Peter N.;Dufrene, Yves F.
通讯作者：
Dufrene, Yves F.