Efficacy of a live attenuated vaccine in classical swine fever virus postnatally persistently infected pigs.
Efficacy of a live attenuated vaccine in classical swine fever virus postnatally persistently infected pigs.
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DOI：
10.1186/s13567-015-0209-9
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发表时间：
2015-07-09
影响因子：
4.4
通讯作者：
Ganges L
中科院分区：
文献类型：
--
作者：
Muñoz-González S;Perez-Simó M;Muñoz M;Bohorquez JA;Rosell R;Summerfield A;Domingo M;Ruggli N;Ganges L
猪瘟（Classical Swine Fever，CSF）是一种严重程度不一的疾病，给养猪业造成重大损失.猪瘟病毒（CSFV）的有效减毒活疫苗在流行国家中常规使用。然而，尽管在这些地区实施了20多年的强化疫苗接种计划，但CSF仍未根除。这些地区的分子流行病学研究表明，该领域传播的病毒在对疫苗的免疫反应施加的正选择压力下发生了进化，产生了新的减毒病毒变种。我们小组最近的工作表明，高比例的持续感染仔猪可以通过出生后早期感染低和中等毒力的CSFV毒株产生。在这里，我们研究了猪瘟兔化弱毒疫苗（HCLV），C株，在6周龄持续感染的猪出生后感染的免疫反应。接种CSFV阴性猪作为对照。在疫苗接种后21天监测体液和干扰素γ应答以及CSFV RNA载量。在接种后21天内，在来自CSFV出生后持续感染猪的血清样品和扁桃体中未检测到疫苗病毒RNA。此外，在CSFV持续感染的接种动物中未显示E2特异性抗体应答或中和抗体滴度。同样，没有观察到针对CSFV或PHA的IFN-γ产生细胞应答。据我们所知，这是第一份证明CSFV持续感染猪对疫苗接种无应答的报告。
Classical swine fever (CSF) causes major losses in pig farming, with various degrees of disease severity. Efficient live attenuated vaccines against classical swine fever virus (CSFV) are used routinely in endemic countries. However, despite intensive vaccination programs in these areas for more than 20 years, CSF has not been eradicated. Molecular epidemiology studies in these regions suggests that the virus circulating in the field has evolved under the positive selection pressure exerted by the immune response to the vaccine, leading to new attenuated viral variants. Recent work by our group demonstrated that a high proportion of persistently infected piglets can be generated by early postnatal infection with low and moderately virulent CSFV strains. Here, we studied the immune response to a hog cholera lapinised virus vaccine (HCLV), C-strain, in six-week-old persistently infected pigs following post-natal infection. CSFV-negative pigs were vaccinated as controls. The humoral and interferon gamma responses as well as the CSFV RNA loads were monitored for 21 days post-vaccination. No vaccine viral RNA was detected in the serum samples and tonsils from CSFV postnatally persistently infected pigs for 21 days post-vaccination. Furthermore, no E2-specific antibody response or neutralising antibody titres were shown in CSFV persistently infected vaccinated animals. Likewise, no of IFN-gamma producing cell response against CSFV or PHA was observed. To our knowledge, this is the first report demonstrating the absence of a response to vaccination in CSFV persistently infected pigs.
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影响因子：
3.7
作者：
Graham SP;Everett HE;Haines FJ;Johns HL;Sosan OA;Salguero FJ;Clifford DJ;Steinbach F;Drew TW;Crooke HR
通讯作者：
Crooke HR
影响因子：
3.2
作者：
Ji, Wei;Niu, Dan-Dan;He, Cheng-Qiang
通讯作者：
He, Cheng-Qiang
影响因子：
5.4
作者：
Lee, YM;Tscherne, DM;Rice, CM
通讯作者：
Rice, CM
影响因子：
5
作者：
de Arce, HD;Ganges, L;Núñez, JI
通讯作者：
Núñez, JI
DOI：
10.1111/j.1439-0450.2004.00766.x
发表时间：
2004-08-01
期刊：
JOURNAL OF VETERINARY MEDICINE SERIES B-INFECTIOUS DISEASES AND VETERINARY PUBLIC HEALTH
影响因子：
--
作者：
Kaden, V;Lange, E;Lange, B
通讯作者：
Lange, B