Challenge of pigs with classical swine fever viruses after C-strain vaccination reveals remarkably rapid protection and insights into early immunity.
Challenge of pigs with classical swine fever viruses after C-strain vaccination reveals remarkably rapid protection and insights into early immunity.
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DOI：
10.1371/journal.pone.0029310
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发表时间：
2012
期刊：
影响因子：
3.7
通讯作者：
Crooke HR
中科院分区：
文献类型：
--
作者：
Graham SP;Everett HE;Haines FJ;Johns HL;Sosan OA;Salguero FJ;Clifford DJ;Steinbach F;Drew TW;Crooke HR
先发制人的扑杀作为控制包括猪瘟（CSF）在内的动物疾病的一种手段越来越受到质疑。这引发了关于使用紧急疫苗来控制未来家猪中CSF爆发的讨论。尽管在流行地区安全使用的历史很长，但关于紧急战略的重要方面的数据很少，例如CSFV疫苗如何迅速防止传播，以及这种保护是否对所有病毒基因型都是等效的，包括高度不同的基因型3株。为评价这些问题，在用基因型2.1和3.3攻毒株攻毒前1、3和5天，对猪接种Riemser® C毒株疫苗。该疫苗对两种攻毒株引起的临床疾病提供了等效的保护，正如预期的那样，在接种后5天完成保护。3天后达到了实质性保护，足以防止3.3菌株传播给直接接触的动物。即使在接种后一天，也有大约一半的动物受到部分保护，并且能够控制感染，这表明接种后感染潜力非常迅速地降低。T细胞IFN-γ应答与保护作用之间存在密切的时间相关性。有趣的是，与接种后5天攻毒动物的应答相比，接种后3天或1天攻毒动物导致疫苗诱导的T细胞应答受损。这与在未保护和未接种的动物中未能检测到T细胞IFN-γ应答一起表明，强毒CSFV可在接种后早期抑制由C-毒株引发的有效抗病毒宿主防御。
Pre-emptive culling is becoming increasingly questioned as a means of controlling animal diseases, including classical swine fever (CSF). This has prompted discussions on the use of emergency vaccination to control future CSF outbreaks in domestic pigs. Despite a long history of safe use in endemic areas, there is a paucity of data on aspects important to emergency strategies, such as how rapidly CSFV vaccines would protect against transmission, and if this protection is equivalent for all viral genotypes, including highly divergent genotype 3 strains. To evaluate these questions, pigs were vaccinated with the Riemser® C-strain vaccine at 1, 3 and 5 days prior to challenge with genotype 2.1 and 3.3 challenge strains. The vaccine provided equivalent protection against clinical disease caused by for the two challenge strains and, as expected, protection was complete at 5 days post-vaccination. Substantial protection was achieved after 3 days, which was sufficient to prevent transmission of the 3.3 strain to animals in direct contact. Even by one day post-vaccination approximately half the animals were partially protected, and were able to control the infection, indicating that a reduction of the infectious potential is achieved very rapidly after vaccination. There was a close temporal correlation between T cell IFN-γ responses and protection. Interestingly, compared to responses of animals challenged 5 days after vaccination, challenge of animals 3 or 1 days post-vaccination resulted in impaired vaccine-induced T cell responses. This, together with the failure to detect a T cell IFN-γ response in unprotected and unvaccinated animals, indicates that virulent CSFV can inhibit the potent antiviral host defences primed by C-strain in the early period post vaccination.
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影响因子：
2.6
作者：
Mourits, M. C. M.;van Asseldonk, M. A. P. M.;Huirne, R. B. M.
通讯作者：
Huirne, R. B. M.
影响因子：
2.7
作者：
Pan, CH;Jong, MH;Lai, SS
通讯作者：
Lai, SS
影响因子：
3.3
作者：
Koenig, Patricia;Hoffmann, Bernd;Beer, Martin
通讯作者：
Beer, Martin
影响因子：
3.3
作者：
BIRONT, P;LEUNEN, J;VANDEPUTTE, J
通讯作者：
VANDEPUTTE, J
影响因子：
3.3
作者：
Floegel-Niesmann, G
通讯作者：
Floegel-Niesmann, G