Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen.
Characterization of PR-10 genes from eight Betula species and detection of Bet v 1 isoforms in birch pollen.
复制标题
DOI：
10.1186/1471-2229-9-24
复制
发表时间：
2009-03-03
影响因子：
5.3
通讯作者：
Gilissen LJ
中科院分区：
文献类型：
--
作者：
Schenk MF;Cordewener JH;America AH;Van't Westende WP;Smulders MJ;Gilissen LJ
Bet v 1是北方欧洲花粉热的一个重要原因。来自欧洲白色桦树（Betula pendula）的Bet v 1亚型已被广泛研究，但其他桦树物种的致敏效力尚不清楚。Bet v 1和密切相关的PR-10基因在基因组中的存在，建立了来自8个桦树种，代表属内的4个亚属的等位基因的扩增和测序。应用Q-TOF LC-MSE来鉴定哪些PR-10/Bet v 1基因实际上在花粉中表达，并确定花粉蛋白质组中各个同种型的相对丰度。所有被检测的桦树物种都含有几个PR-10基因。总共回收了134个独特序列。序列被归因于不同的基因或假基因，这些基因或假基因依次被分为七个亚家族。五个亚科是共同的所有桦树种。两个亚科的基因在花粉中表达，而每个桦树品种表达的异构体的混合物，至少有四个不同的异构体。除B外，所有种属中均存在与具有高IgE反应性（Bet v 1a = PR-10.01 A01）的同种型相似的同种型。低变应原性亚型Bet v 1d（= PR-10.01 B 01）仅见于B。pendula及其近亲。Q-TOF LC-MSE允许通过确定花粉中这些同种型的存在和相对丰度来有效筛选Bet v 1同种型。B。pendula含有Bet v 1混合物，其中具有高和低IgE反应性的亚型都很丰富。可能除了B。lenta，同种型相同或非常相似的那些具有高IgE-反应性被发现在花粉蛋白质组的所有检查桦树品种。因此，这些物种也被预测为与Bet v 1相关的过敏原。
Bet v 1 is an important cause of hay fever in northern Europe. Bet v 1 isoforms from the European white birch (Betula pendula) have been investigated extensively, but the allergenic potency of other birch species is unknown. The presence of Bet v 1 and closely related PR-10 genes in the genome was established by amplification and sequencing of alleles from eight birch species that represent the four subgenera within the genus Betula. Q-TOF LC-MSE was applied to identify which PR-10/Bet v 1 genes are actually expressed in pollen and to determine the relative abundances of individual isoforms in the pollen proteome. All examined birch species contained several PR-10 genes. In total, 134 unique sequences were recovered. Sequences were attributed to different genes or pseudogenes that were, in turn, ordered into seven subfamilies. Five subfamilies were common to all birch species. Genes of two subfamilies were expressed in pollen, while each birch species expressed a mixture of isoforms with at least four different isoforms. Isoforms that were similar to isoforms with a high IgE-reactivity (Bet v 1a = PR-10.01A01) were abundant in all species except B. lenta, while the hypoallergenic isoform Bet v 1d (= PR-10.01B01) was only found in B. pendula and its closest relatives. Q-TOF LC-MSE allows efficient screening of Bet v 1 isoforms by determining the presence and relative abundance of these isoforms in pollen. B. pendula contains a Bet v 1-mixture in which isoforms with a high and low IgE-reactivity are both abundant. With the possible exception of B. lenta, isoforms identical or very similar to those with a high IgE-reactivity were found in the pollen proteome of all examined birch species. Consequently, these species are also predicted to be allergenic with regard to Bet v 1 related allergies.
登录
查看更多内容
DOI：
10.1084/jem.183.2.599
发表时间：
1996-02-01
期刊：
The Journal of experimental medicine
影响因子：
--
作者：
Ferreira F;Hirtenlehner K;Jilek A;Godnik-Cvar J;Breiteneder H;Grimm R;Hoffmann-Sommergruber K;Scheiner O;Kraft D;Breitenbach M;Rheinberger HJ;Ebner C
通讯作者：
Ebner C
影响因子：
3.4
作者：
de Groot, Joost C. W.;Fiers, Mark W. E. J.;America, Antoine H. P.
通讯作者：
America, Antoine H. P.
影响因子：
11.4
作者：
BREITENEDER, H;PETTENBURGER, K;BREITENBACH, M
通讯作者：
BREITENBACH, M
影响因子：
9.1
作者：
Abe, Y;Kimura, S;Katagiri, M
通讯作者：
Katagiri, M
影响因子：
12.4
作者：
CADOT, P;LEJOLY, M;STEVENS, EAM
通讯作者：
STEVENS, EAM