A dental implant-on-a-chip for 3D modeling of host-material-pathogen interactions and therapeutic testing platforms.
A dental implant-on-a-chip for 3D modeling of host-material-pathogen interactions and therapeutic testing platforms.
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DOI：
10.1039/d2lc00774f
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发表时间：
2022-12-06
期刊：
影响因子：
6.1
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中科院分区：
文献类型：
--
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芯片实验室设备在小范围内对流体动力学进行精确的时空控制和操纵，为宿主-病原体相互作用的体内研究提供了一个新的生物医学研究领域。虽然人类使用的各种医疗器械/植入物的使用有所增加，但集成此类功能性生物材料的微流体模型的适用性目前受到限制。在这里，我们介绍了一种新的牙科种植体芯片模型，以更好地了解种植体周围疾病背景下的宿主-材料-病原体相互作用。芯片植入物将牙龈细胞与相关生物材料整合在一起-角质形成细胞与牙科树脂和成纤维细胞与钛，同时保持空间分离的共培养。为了实现这种共培养，芯片上植入物的核心结构需要紧密间隔的高微沟槽。因此，通过采用具有选择性滤光器的独特背面UV曝光来创建具有高纵横比柱阵列的SU-8母模。利用该模型，我们成功地复制了牙种植体生物材料附近的角质形成细胞和成纤维细胞的形态。此外，我们证明了光生物调节疗法如何用于保护上皮层免受反复的细菌挑战（与对照相比，细胞损伤减少约3.5倍）。总的来说，我们的牙科植入物芯片方法提出了一种新的微流体模型，用于多重宿主材料病原体调查和评估传染病的新治疗策略。整个牙科种植体微环境成功地复制在微流体模型中，通过前所未有的高纵横比的微通道由紧密间隔的微柱分离。
The precise spatiotemporal control and manipulation of fluid dynamics on a small scale granted by Lab-on-a-Chip devices provide a new biomedical research realm as a substitute for in vivo studies of host-pathogen interactions. While there has been a rise in the use of various medical devices/implants for human use, the applicability of microfluidic models that integrate such functional biomaterials is currently limited. Here, we introduced a novel dental implant-on-a-chip model to better understand host-material-pathogen interactions in the context of peri-implant diseases. The implant-on-a-chip integrates gingival cells with relevant biomaterials - keratinocytes with dental resin and fibroblasts with titanium while maintaining a spatially separated co-culture. To enable this co-culture, the implant-on-a-chip's core structure necessitates closely spaced, tall microtrenches. Thus, a SU-8 master mold with a high aspect-ratio pillar array was created by employing a unique backside UV exposure with a selective optical filter. With this model, we successfully replicated the morphology of keratinocytes and fibroblasts in the vicinity of dental implant biomaterials. Furthermore, we demonstrated how photobiomodulation therapy might be used to protect the epithelial layer from recurrent bacterial challenges (~3.5-fold reduction in cellular damage vs. control). Overall, our dental implant-on-a-chip approach proposes a new microfluidic model for multiplexed host-material-pathogen investigations and the evaluation of novel treatment strategies for infectious diseases. The entire dental implant microenvironment is successfully replicated in the microfluidic model via unprecedented high-aspect-ratio microchannels separated by closely-spaced micropillars.
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影响因子：
2.9
作者：
Agarwal, Tarun;Narayana, Gautham H.;Banerjee, Indranil
通讯作者：
Banerjee, Indranil
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Phillips, K. Scott
影响因子：
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作者：
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影响因子：
2.8
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通讯作者：
Luo X