Extensively duplicated and transcriptionally active recent lateral gene transfer from a bacterial Wolbachia endosymbiont to its host filarial nematode Brugia malayi.
Extensively duplicated and transcriptionally active recent lateral gene transfer from a bacterial Wolbachia endosymbiont to its host filarial nematode Brugia malayi.
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DOI：
10.1186/1471-2164-14-639
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发表时间：
2013-09-22
期刊：
影响因子：
4.4
通讯作者：
Dunning Hotopp JC
中科院分区：
文献类型：
--
作者：
Ioannidis P;Johnston KL;Riley DR;Kumar N;White JR;Olarte KT;Ott S;Tallon LJ;Foster JM;Taylor MJ;Dunning Hotopp JC
淋巴丝虫病是一种被忽视的热带疾病，影响着1.2亿多人，另有13亿人面临感染风险。马来丝虫（Brugia malayi）是该病的病原体之一，与沃尔巴克氏体（Wolbachia）细菌共生。由于广泛的横向基因转移经常发生在沃尔巴克氏体和它的宿主之间，我们试图测量这种LGT在B中的程度。通过对沃尔巴克氏体耗尽蠕虫的全基因组测序，一个相当大的部分（至少115.4 kbp，或10.6%）的1.08 Mbp的沃尔巴克氏体wBm基因组已转移到其线虫宿主，并保留了高水平的相似性，包括227 wBm基因和基因片段。其中32个基因的完整开放阅读框被转移，这意味着它们有可能产生功能蛋白质。此外，有证据表明，四个转让的生活阶段特异性转录调节水平类似于其他线虫转录本，加强了他们的功能的可能性。沃尔巴克氏体DNA广泛且持续地转移到蠕虫基因组中，并且一些转移以阶段特异性方式在生物学相关水平上转录。
Lymphatic filariasis is a neglected tropical disease afflicting more than 120 million people, while another 1.3 billion people are at risk of infection. The nematode worm Brugia malayi is one of the causative agents of the disease and exists in a mutualistic symbiosis with Wolbachia bacteria. Since extensive lateral gene transfer occurs frequently between Wolbachia and its hosts, we sought to measure the extent of such LGT in B. malayi by whole genome sequencing of Wolbachia-depleted worms. A considerable fraction (at least 115.4-kbp, or 10.6%) of the 1.08-Mbp Wolbachia wBm genome has been transferred to its nematode host and retains high levels of similarity, including 227 wBm genes and gene fragments. Complete open reading frames were transferred for 32 of these genes, meaning they have the potential to produce functional proteins. Moreover, four transfers have evidence of life stage-specific regulation of transcription at levels similar to other nematode transcripts, strengthening the possibility that they are functional. There is extensive and ongoing transfer of Wolbachia DNA to the worm genome and some transfers are transcribed in a stage-specific manner at biologically relevant levels.
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影响因子：
30.8
作者：
Desjardins, Christopher A.;Cerqueira, Gustavo C.;Goldberg, Jonathan M.;Hotopp, Julie C. Dunning;Haas, Brian J.;Zucker, Jeremy;Ribeiro, Jose M. C.;Saif, Sakina;Levin, Joshua Z.;Fan, Lin;Zeng, Qiandong;Russ, Carsten;Wortman, Jennifer R.;Fink, Doran L.;Birren, Bruce W.;Nutman, Thomas B.
通讯作者：
Nutman, Thomas B.
影响因子：
56.9
作者：
Hotopp, Julie C. Dunning;Clark, Michael E.;Werren, John H.
通讯作者：
Werren, John H.
影响因子：
11.4
作者：
Hotopp, Julie C. Dunning
通讯作者：
Hotopp, Julie C. Dunning
影响因子：
5.2
作者：
Lin M;Kikuchi T;Brewer HM;Norbeck AD;Rikihisa Y
通讯作者：
Rikihisa Y
DOI：
10.1073/pnas.1011481108
发表时间：
2011-06-07
影响因子：
11.1
作者：
Bennuru, Sasisekhar;Meng, Zhaojing;Nutman, Thomas B.
通讯作者：
Nutman, Thomas B.