Genomics of Loa loa, a Wolbachia-free filarial parasite of humans.
Genomics of Loa loa, a Wolbachia-free filarial parasite of humans.
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DOI：
10.1038/ng.2585
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发表时间：
2013-05
期刊：
影响因子：
30.8
通讯作者：
Nutman, Thomas B.
中科院分区：
文献类型：
--
作者：
Desjardins, Christopher A.;Cerqueira, Gustavo C.;Goldberg, Jonathan M.;Hotopp, Julie C. Dunning;Haas, Brian J.;Zucker, Jeremy;Ribeiro, Jose M. C.;Saif, Sakina;Levin, Joshua Z.;Fan, Lin;Zeng, Qiandong;Russ, Carsten;Wortman, Jennifer R.;Fink, Doran L.;Birren, Bruce W.;Nutman, Thomas B.
Loa loa，非洲眼虫，是人类的主要丝虫病原体。与大多数丝虫不同，Loa loa不包含专性细胞内Wolbachia内共生体。我们描述了Loa loa的91.4 Mb基因组和相关丝虫寄生虫Wuchereria bancrofti的基因组，并基于微丝蚴RNA测序预测了14，907个Loa loa基因。通过比较这些基因组的另一种丝虫寄生虫，马来丝虫，和其他几个线虫基因组，我们证明了共线性丝虫之间，但不是与非寄生线虫。Loa loa基因组编码许多免疫相关基因，以及目前批准用于人类的药物靶向的蛋白激酶。尽管缺乏沃尔巴克氏体，Loa loa与其他丝虫相比没有显示新的代谢合成或运输能力。这些结果表明，沃尔巴克氏体在丝虫生物学中所起的作用比以前认为的更微妙，并揭示了寄生线虫和非寄生线虫之间的显着差异。
Loa loa, the African eyeworm, is a major filarial pathogen of humans. Unlike most filariae, Loa loa does not contain the obligate intracellular Wolbachia endosymbiont. We describe the 91.4 Mb genome of Loa loa, and the genome of the related filarial parasite Wuchereria bancrofti, and predict 14,907 Loa loa genes based on microfilarial RNA sequencing. By comparing these genomes to that of another filarial parasite, Brugia malayi, and to several other nematode genomes, we demonstrate synteny among filariae but not with non-parasitic nematodes. The Loa loa genome encodes many immunologically relevant genes, as well as protein kinases targeted by drugs currently approved for humans. Despite lacking Wolbachia, Loa loa shows no new metabolic synthesis or transport capabilities compared to other filariae. These results suggest that the role played by Wolbachia in filarial biology is more subtle than previously thought and reveal marked differences between parasitic and non-parasitic nematodes.
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