Screening of Microorganisms for Biodegradation of Simazine Pollution (Obsolete Pesticide Azotop 50 WP).

Screening of Microorganisms for Biodegradation of Simazine Pollution (Obsolete Pesticide Azotop 50 WP).
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DOI:
10.1007/s11270-011-0761-5
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发表时间:
2011-09
影响因子:
2.9
通讯作者:
Graczyk, Paulina
Graczyk, Paulina
中科院分区:
环境科学与生态学4区
文献类型:
--
作者:
Blaszak, Magdalena;Pelech, Robert;Graczyk, Paulina

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评估了环境微生物生物降解2-氯-s-三嗪除草剂(自2007年以来的农药废物)的活性物质的能力。微生物巨大的代谢潜力促使人们探索利用它们作为热和化学利用方法的替代方法的可能性。首先,研究了富耐辛微生物的生物群落。只有较高剂量(100 mg/l)的西马辛对底物培养的细菌和环境真菌的数量有实际影响。活性污泥和生物腐殖质(蚯蚓堆肥)中大多数耐辛细菌;在玉米花土和玉米层土中发现了最大的抗性菌种。堆肥和生物腐殖质是生物降解西马辛的微生物来源,但考虑到西马辛抗性微生物的数量,两者都是优势。在两种周期性培养的情况下(来自生物腐殖质和堆肥的微生物),近100%的西马辛(50 mg/l)被降解(在8天内)。反复富集培养后,辛马嗪的降解速度大大加快,仅在24 h后,辛马嗪的降解量显著下降(堆肥中下降20%,生物腐殖质中下降80%)。虽然进行了十几次从堆肥和生物腐殖质中分离出各种负责生物降解西马辛的菌株的尝试,但只有一株菌株被鉴定为关节杆菌(NC),它的生物降解效率几乎是100%(在4天内)。
The capability of environmental microorganisms to biodegrade simazine—an active substance of 2-chloro-s-triazine herbicides (pesticide waste since 2007)—was assessed. An enormous metabolic potential of microorganisms impels to explore the possibilities of using them as an alternative way for thermal and chemical methods of utilization. First, the biotope rich in microorganisms resistant to simazine was examined. Only the higher dose of simazine (100 mg/l) had an actual influence on quantity of bacteria and environmental fungi incubated on substrate with simazine. Most simazine-resistant bacteria populated activated sludge and biohumus (vermicompost); the biggest strain of resistant fungi was found in floral soil and risosphere soil of maize. Compost and biohumus were the sources of microorganisms which biodegraded simazine, though either of them was the dominant considering the quantity of simazine-resistant microorganisms. In both cases of periodic culture (microorganisms from biohumus and compost), nearly 100% of simazine (50 mg/l) was degraded (within 8 days). After the repeated enrichment culture with simazine, the rate of its degradation highly accelerated, and just after 24 h, the significant decrease of simazine (20% in compost and 80% in biohumus) was noted. Although a dozen attempts of isolating various strains responsible for biodegradation of simazine from compost and biohumus were performed, only the strain identified as Arthrobacter urefaciens (NC) was obtained, and it biodegraded simazine with almost 100% efficiency (within 4 days).
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