Antitumor activity of human hydatid cyst fluid in a murine model of colon cancer.
Antitumor activity of human hydatid cyst fluid in a murine model of colon cancer.
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DOI：
10.1155/2013/230176
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发表时间：
2013
影响因子：
--
通讯作者：
Osinaga E
中科院分区：
文献类型：
--
作者：
Berriel E;Russo S;Monin L;Festari MF;Berois N;Fernández G;Freire T;Osinaga E
本研究在结肠癌动物模型中评价人囊液（HCF）诱导的抗肿瘤免疫反应。我们发现抗hcf抗体能够识别CT26结肠癌细胞的细胞表面和细胞内抗原。在预防性肿瘤攻击实验中，发现HCF疫苗接种对40%的小鼠的肿瘤形成有保护作用（P = 0.01）。在治疗组，HCF疫苗接种诱导40%的接种小鼠肿瘤消退（P = 0.05）。这种疫苗产生了记忆免疫反应，保护存活的小鼠免受肿瘤的再次攻击，暗示在这一过程中发生了适应性免疫反应。我们对抗HCF抗体识别的CT26抗原进行了蛋白质组学分析，以分析大肠杆菌颗粒（HCF）和CT26结肠癌细胞之间的免疫交叉反应性。我们确定了两种蛋白：mortalin和肌酸激酶m型。有趣的是，CT26 mortalin与E. granulosus hsp70具有60%的同源性。总之，我们的数据表明，在动物模型中，HCF疫苗接种能够诱导抗肿瘤免疫，从而防止肿瘤生长。这一新的抗肿瘤策略为高免疫原性抗癌疫苗的开发开辟了新的视野。
This study evaluates the antitumor immune response induced by human hydatic cyst fluid (HCF) in an animal model of colon carcinoma. We found that anti-HCF antibodies were able to identify cell surface and intracellular antigens in CT26 colon cancer cells. In prophylactic tumor challenge experiments, HCF vaccination was found to be protective against tumor formation for 40% of the mice (P = 0.01). In the therapeutic setting, HCF vaccination induced tumor regression in 40% of vaccinated mice (P = 0.05). This vaccination generated memory immune responses that protected surviving mice from tumor rechallenge, implicating the development of an adaptive immune response in this process. We performed a proteomic analysis of CT26 antigens recognized by anti-HCF antibodies to analyze the immune cross-reactivity between E. granulosus (HCF) and CT26 colon cancer cells. We identified two proteins: mortalin and creatine kinase M-type. Interestingly, CT26 mortalin displays 60% homology with E. granulosus hsp70. In conclusion, our data demonstrate the capacity of HCF vaccination to induce antitumor immunity which protects from tumor growth in an animal model. This new antitumor strategy could open new horizons in the development of highly immunogenic anticancer vaccines.
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DOI：
10.4049/jimmunol.1201209
发表时间：
2013-01-01
期刊：
Journal of immunology (Baltimore, Md. : 1950)
影响因子：
--
作者：
Baird JR;Byrne KT;Lizotte PH;Toraya-Brown S;Scarlett UK;Alexander MP;Sheen MR;Fox BA;Bzik DJ;Bosenberg M;Mullins DW;Turk MJ;Fiering S
通讯作者：
Fiering S
影响因子：
4.6
作者：
Osinaga, Eduardo
通讯作者：
Osinaga, Eduardo
影响因子：
9.7
作者：
PIDHERNEY, MS;ALIZADEH, H;NIEDERKORN, JY
通讯作者：
NIEDERKORN, JY
影响因子：
4.6
作者：
RIGANO, R;PROFUMO, E;SIRACUSANO, A
通讯作者：
SIRACUSANO, A
影响因子：
4.6
作者：
RIGANO, R;PROFUMO, E;SIRACUSANO, A
通讯作者：
SIRACUSANO, A