Gene profiling of narrowband UVB-induced skin injury defines cellular and molecular innate immune responses.
Gene profiling of narrowband UVB-induced skin injury defines cellular and molecular innate immune responses.
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DOI：
10.1038/jid.2012.359
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发表时间：
2013-03
影响因子：
6.5
通讯作者：
Krueger, James G.
中科院分区：
文献类型：
--
作者：
Crispin, Milene Kennedy;Fuentes-Duculan, Judilyn;Gulati, Nicholas;Johnson-Huang, Leanne M.;Lentini, Tim;Sullivan-Whalen, Mary;Gilleaudeau, Patricia;Cueto, Inna;Suarez-Farinas, Mayte;Lowes, Michelle A.;Krueger, James G.
人体皮肤对紫外线B(UVB)辐射的急性反应尚未完全确定。我们试图使用转录图谱、免疫组织化学和免疫荧光来确定窄带UVB(NB-UVB，312 nm峰值)后24小时的皮肤反应，窄带UVB是治疗相关的UVB来源。有1,522个独特的差异表达基因，包括抗菌肽(AMPs)(S100A7、S100A12、人β-防御素2和弹力素)、中性粒细胞和单核细胞/树突状细胞(DC)趋化因子上调(IL-8、CXCL1、CCL20、CCL2)。独创性通路分析表明，先天防御和早期获得性免疫通路被激活。免疫组织化学证实表皮AMPs(S100A7、S100A12、人β-防御素2和弹力素)染色增加。受照皮肤CD11c+BDCA1−树突状细胞增多，与DC-LAMP最小共定位显示未成熟的DC，共表达炎症标志物肿瘤坏死因子和TRAIL。BDCA3+DC增加，这是一种具有免疫抑制功能的交叉呈递DC亚型，这些细胞以前没有被描述为UVB反应的一部分。这些结果表明，人体皮肤对NB-UVB致红斑剂量的急性反应包括天然防御机制的激活，以及多种亚型炎性DC的早期渗透，这可能是天然免疫和获得性免疫之间的联系。
The acute response of human skin to ultraviolet B (UVB) radiation has not been fully characterized. We sought to define the cutaneous response at 24 hours following narrow-band UVB (NB-UVB, 312 nm peak), a therapeutically relevant source of UVB, using transcriptional profiling, immunohistochemistry, and immunofluorescence. There were 1,522 unique differentially-regulated genes, including upregulation of antimicrobial peptides (AMPs) (S100A7, S100A12, human beta-defensin 2, and elafin), neutrophil and monocyte/dendritic cell (DC) chemoattractants (IL-8, CXCL1, CCL20, CCL2). Ingenuity Pathway Analysis demonstrated activation of innate defense and early adaptive immune pathways. Immunohistochemistry confirmed increased epidermal staining for AMPs (S100A7, S100A12, human beta-defensin 2, and elafin). Inflammatory myeloid CD11c+BDCA1− DCs were increased in irradiated skin, which were immature as shown by minimal co-localization with DC-LAMP, and co-expressed inflammatory markers TNF and TRAIL in irradiated skin. There were increased BDCA3+ DCs, a cross-presenting DC subtype with immunosuppressive functions, and these cells have not been previously characterized as part of the response to UVB. These results show that the acute response of human skin to erythemogenic doses of NB-UVB includes activation of innate defense mechanisms, as well as early infiltration of multiple subtypes of inflammatory DCs, which could serve as a link between innate and adaptive immunity.
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影响因子：
6.5
作者：
Kennedy-Crispin, Milene;Billick, Erika;Mitsui, Hiroshi;Gulati, Nicholas;Fujita, Hideki;Gilleaudeau, Patricia;Sullivan-Whalen, Mary;Johnson-Huang, Leanne M.;Suarez-Farinas, Mayte;Krueger, James G.
通讯作者：
Krueger, James G.
影响因子：
3.2
作者：
Broome, AM;Ryan, D;Eckert, RL
通讯作者：
Eckert, RL
影响因子：
6.5
作者：
Johnson-Huang, Leanne M.;Suarez-Farinas, Mayte;Pierson, Katherine C.;Fuentes-Duculan, Judilyn;Cueto, Irma;Lentini, Tim;Sullivan-Whalen, Mary;Gilleaudeau, Patricia;Krueger, James G.;Haider, Asifa S.;Lowes, Michelle A.
通讯作者：
Lowes, Michelle A.
DOI：
10.1073/pnas.74.4.1688
发表时间：
1977-01-01
影响因子：
11.1
作者：
FISHER, MS;KRIPKE, ML
通讯作者：
KRIPKE, ML
DOI：
10.1073/pnas.0409569102
发表时间：
2005-02-08
影响因子：
11.1
作者：
Chamian, F;Lowes, MA;Krueger, JG
通讯作者：
Krueger, JG