An immune evasion mechanism for spirochetal persistence in Lyme borreliosis.
An immune evasion mechanism for spirochetal persistence in Lyme borreliosis.
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DOI：
10.1084/jem.20011870
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发表时间：
2002-02-18
期刊：
影响因子：
--
通讯作者：
Philipp MT
中科院分区：
文献类型：
--
作者：
Liang FT;Jacobs MB;Bowers LC;Philipp MT
莱姆病螺旋体伯氏疏螺旋体，持续感染哺乳动物宿主，尽管针对病原体的强烈体液应答的发展。在这里，我们描述了一种新的免疫逃避机制的B。burgdorferi。在免疫功能正常的小鼠中，在接种后17天内选择不表达ospC（外表面蛋白C基因）的螺旋体，伴随着抗OspC抗体的出现。从免疫活性小鼠中分离的没有检测到OspC转录物的螺旋体在体外培养或移植到幼稚免疫活性小鼠后重新表达ospC，但在OspC免疫小鼠中不表达。B。burgdorferi在严重联合免疫缺陷（SCID）小鼠中持续表达ospC。B被动免疫。用抗OspC单克隆抗体感染burgdorferi的SCID小鼠选择性地消除表达OspC的螺旋体，但不清除感染。在抗OspC抗体消除后，表达OspC的螺旋体在SCID小鼠中重新出现。我们认为选择表面抗原nonexpressers是一种免疫逃避机制，有助于螺旋体的持久性。
Borrelia burgdorferi, the Lyme disease spirochete, persistently infects mammalian hosts despite the development of strong humoral responses directed against the pathogen. Here we describe a novel mechanism of immune evasion by B. burgdorferi. In immunocompetent mice, spirochetes that did not express ospC (the outer-surface protein C gene) were selected within 17 d after inoculation, concomitantly with the emergence of anti-OspC antibody. Spirochetes with no detectable OspC transcript that were isolated from immunocompetent mice reexpressed ospC after they were either cultured in vitro or transplanted to naive immunocompetent mice, but not in OspC-immunized mice. B. burgdorferi persistently expressed ospC in severe combined immune-deficient (SCID) mice. Passive immunization of B. burgdorferi–infected SCID mice with an anti-OspC monoclonal antibody selectively eliminated ospC-expressing spirochetes but did not clear the infection. OspC-expressing spirochetes reappeared in SCID mice after the anti-OspC antibody was eliminated. We submit that selection of surface-antigen nonexpressers is an immune evasion mechanism that contributes to spirochetal persistence.
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影响因子：
3.1
作者：
Brown, JP;Zachary, JF;Wooten, RM
通讯作者：
Wooten, RM
影响因子：
3.1
作者：
Gilmore, RD;Kappel, KJ;Johnson, BJB
通讯作者：
Johnson, BJB
影响因子：
3.1
作者：
NGUYEN, TPK;LAM, TT;FIKRIG, E
通讯作者：
FIKRIG, E
DOI：
10.1073/pnas.231442498
发表时间：
2001-10-23
影响因子：
11.1
作者：
Hübner, A;Yang, XF;Norgard, MV
通讯作者：
Norgard, MV
影响因子：
4.4
作者：
Fikrig, E;Feng, W;Flavell, RA
通讯作者：
Flavell, RA