Large-Scale Plasma Peptidomic Profiling Reveals a Novel, Nontoxic, Crassostrea hongkongensis-Derived Antimicrobial Peptide against Foodborne Pathogens.
Large-Scale Plasma Peptidomic Profiling Reveals a Novel, Nontoxic, Crassostrea hongkongensis-Derived Antimicrobial Peptide against Foodborne Pathogens.
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大规模血浆肽组学分析揭示了一种新型、无毒、源自香港牡蛎的抗食源性病原体抗菌肽
DOI：
10.3390/md19080420
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发表时间：
2021-07-26
期刊：
影响因子：
5.4
通讯作者：
Zhang Y
中科院分区：
文献类型：
--
作者：
Mao F;Bao Y;Wong NK;Huang M;Liu K;Zhang X;Yang Z;Yi W;Shu X;Xiang Z;Yu Z;Zhang Y
抗菌肽是软体动物防御系统的基本组成部分，尽管它们仍然是一个很少研究的课题。在这里，副溶血性弧菌感染引发了牡蛎血浆中抗菌活性的显着增加。通过使用PBS攻击的牡蛎作为对照，来自免疫攻击的牡蛎的血浆肽进行肽组学分析和计算机数据挖掘以鉴定生物活性肽。35个鉴定的血浆肽在感染后上调，其中6个上调肽（URPs）显示相对高的正电荷。URP20经验证具有显著的抗菌活性。实际上，URP20引发细菌细胞的聚集，伴随着它们的膜透化。有趣的是，URP20被发现对革兰氏阳性和革兰氏阴性食源性病原体以及白色念珠菌具有活性，对哺乳动物细胞和小鼠没有细胞毒性。我们的研究提供了第一个大规模的血浆肽组数据集，确定新的生物活性肽在海洋软体动物。进一步探索海洋无脊椎动物中的肽多样性，将为设计具有广泛应用的新型抗菌肽提供一个富有成效的追求。
Antimicrobial peptides are a fundamental component of mollusks’ defense systems, though they remain a thinly investigated subject. Here, infection by Vibrio parahemolyticus triggered a significant increase in antimicrobial activity in oyster plasma. By using PBS-challenged oysters as a control, plasma peptides from immunologically challenged oysters were subjected to peptidomic profiling and in silico data mining to identify bioactive peptides. Thirty-five identified plasma peptides were up-regulated post infection, among which, six up-regulated peptides (URPs) showed a relatively high positive charge. URP20 was validated with significant antibacterial activity. Virtually, URP20 triggered aggregation of bacterial cells, accompanied by their membrane permeabilization. Interestingly, URP20 was found to be active against Gram-positive and Gram-negative foodborne pathogens as well as Candida albicans, with no cytotoxicity to mammalian cells and mice. Our study provides the first large-scale plasma peptidomic dataset that identifies novel bioactive peptides in marine mollusks. Further exploration of peptide diversity in marine invertebrates should prove a fruitful pursuit for designing novel AMPs with broad applications.
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影响因子：
3.4
作者：
Campos, Alexandre;Apraiz, Itxaso;Cristobal, Susana
通讯作者：
Cristobal, Susana
影响因子：
4.7
作者：
De Zoysa, Mahanama;Whang, Ilson;Lee, Jehee
通讯作者：
Lee, Jehee
白色念珠菌生物膜和人类疾病。
影响因子：
10.5
作者：
Nobile CJ;Johnson AD
通讯作者：
Johnson AD
影响因子：
5.5
作者：
González García M;Rodríguez A;Alba A;Vázquez AA;Morales Vicente FE;Pérez-Erviti J;Spellerberg B;Stenger S;Grieshober M;Conzelmann C;Münch J;Raber H;Kubiczek D;Rosenau F;Wiese S;Ständker L;Otero-González A
通讯作者：
Otero-González A
影响因子：
4.7
作者：
De Zoysa, Mahanama;Nikapitiya, Chamilani;Lee, Jehee
通讯作者：
Lee, Jehee