Vaccination with recombinant aspartic hemoglobinase reduces parasite load and blood loss after hookworm infection in dogs.
Vaccination with recombinant aspartic hemoglobinase reduces parasite load and blood loss after hookworm infection in dogs.
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重组天冬氨酸血红蛋白酶的疫苗接种可减少狗钩虫感染后的寄生虫负荷和失血。
DOI：
10.1371/journal.pmed.0020295
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发表时间：
2005-10
期刊：
影响因子：
15.8
通讯作者：
Hotez, PJ
中科院分区：
文献类型：
--
作者：
Loukas, A;Bethony, JM;Mendez, S;Fujiwara, RT;Goud, GN;Ranjit, N;Zhan, B;Jones, K;Bottazzi, ME;Hotez, PJ
Hookworms infect 730 million people in developing countries where they are a leading cause of intestinal blood loss and iron-deficiency anemia. At the site of attachment to the host, adult hookworms ingest blood and lyse the erythrocytes to release hemoglobin. The parasites subsequently digest hemoglobin in their intestines using a cascade of proteolysis that begins with the Ancylostoma caninum aspartic protease 1, APR-1. We show that vaccination of dogs with recombinant Ac-APR-1 induced antibody and cellular responses and resulted in significantly reduced hookworm burdens (p = 0.056) and fecal egg counts (p = 0.018) in vaccinated dogs compared to control dogs after challenge with infective larvae of A. caninum. Most importantly, vaccinated dogs were protected against blood loss (p = 0.049) and most did not develop anemia, the major pathologic sequela of hookworm disease. IgG from vaccinated animals decreased the catalytic activity of the recombinant enzyme in vitro and the antibody bound in situ to the intestines of worms recovered from vaccinated dogs, implying that the vaccine interferes with the parasite's ability to digest blood. To the best of our knowledge, this is the first report of a recombinant vaccine from a hematophagous parasite that significantly reduces both parasite load and blood loss, and it supports the development of APR-1 as a human hookworm vaccine. Vaccination of dogs with a recombinant protease produced by hookworms can reduce blood loss when these dogs are infected with the hookworm Ancylostoma caninum.
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影响因子：
4
作者：
Girod, N;Brown, A;Billett, EE
通讯作者：
Billett, EE
影响因子：
6.4
作者：
Loukas, A;Bethony, JM;Hotez, PJ
通讯作者：
Hotez, PJ
DOI：
10.1016/0035-9203(95)90101-9
发表时间：
1995-09-01
影响因子：
2.2
作者：
ALBONICO, M;SMITH, PG;SAVIOLI, L
通讯作者：
SAVIOLI, L
影响因子：
1.8
作者：
Boag, PR;Parsons, JC;Sexton, JL
通讯作者：
Sexton, JL
影响因子：
5.5
作者：
Chabalgoity, JA;Moreno, M;Hormaeche, E
通讯作者：
Hormaeche, E