A putative serine protease, SpSsp1, from Saprolegnia parasitica is recognised by sera of rainbow trout, Oncorhynchus mykiss.
A putative serine protease, SpSsp1, from Saprolegnia parasitica is recognised by sera of rainbow trout, Oncorhynchus mykiss.
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DOI：
10.1016/j.funbio.2014.04.008
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发表时间：
2014-07
期刊：
影响因子：
2.5
通讯作者：
Van West, Pieter
中科院分区：
文献类型：
--
作者：
Minor, Kirsty L.;Anderson, Victoria L.;Davis, Katie S.;Van Den Berg, Albert H.;Christie, James S.;Loebach, Lars;Faruk, Ali Reza;Wawra, Stephan;Secombes, Chris J.;Van West, Pieter
腐生菌病是由腐生菌引起的疾病，给水产养殖业造成了相当大的经济损失。目前的控制方法是不充分的，因为它们要么基本上无效，要么存在环境和鱼类健康问题。对鱼类接种疫苗是这些控制方法之外的一种有吸引力的替代方法。因此，我们着手鉴定合适的抗原，以帮助产生一种对抗寄生藻的鱼疫苗。出乎意料的是，在健康虹鳟（Oncorhynchus mykiss）的血清中发现了抗寄生虫的抗体。抗体检测到在一维sds -聚丙烯酰胺凝胶上运行的分泌蛋白质中的单个条带，该条带对应于二维凝胶上的两个蛋白质点。采用液相色谱串联质谱法对蛋白质进行分析。通过Mascot和生物信息学分析，鉴定出含有枯草菌素结构域的481个氨基酸残基的单一分泌蛋白SpSsp1。表达分析表明，SpSsp1在所有被试菌丝期均高表达。对来自英国几个养鱼场的其他未感染鳟鱼的调查显示，它们的血清中对SpSsp1有类似的活性。一些没有明显腐殖质增生的鱼显示出对SpSsp1的抗体反应，这表明SpSsp1可能是未来疫苗接种试验的有用候选者。健康虹鳟鱼血清中有抗寄生虫的抗体。血清与寄生蜂培养滤液中的单一蛋白相互作用。抗原蛋白是一种分泌的枯草杆菌样丝氨酸蛋白酶。
Saprolegniosis, the disease caused by Saprolegnia sp., results in considerable economic losses in aquaculture. Current control methods are inadequate, as they are either largely ineffective or present environmental and fish health concerns. Vaccination of fish presents an attractive alternative to these control methods. Therefore we set out to identify suitable antigens that could help generate a fish vaccine against Saprolegnia parasitica. Unexpectedly, antibodies against S. parasitica were found in serum from healthy rainbow trout, Oncorhynchus mykiss. The antibodies detected a single band in secreted proteins that were run on a one-dimensional SDS-polyacrylamide gel, which corresponded to two protein spots on a two-dimensional gel. The proteins were analysed by liquid chromatography tandem mass spectrometry. Mascot and bioinformatic analysis resulted in the identification of a single secreted protein, SpSsp1, of 481 amino acid residues, containing a subtilisin domain. Expression analysis demonstrated that SpSsp1 is highly expressed in all tested mycelial stages of S. parasitica. Investigation of other non-infected trout from several fish farms in the United Kingdom showed similar activity in their sera towards SpSsp1. Several fish that had no visible saprolegniosis showed an antibody response towards SpSsp1 suggesting that SpSsp1 might be a useful candidate for future vaccination trial experiments. Sera of healthy rainbow trout have antibodies against Saprolegnia parasitica. The sera interact with a single protein from culture filtrate of S. parasitica. The antigenic protein is a secreted subtilisin-like serine protease.
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影响因子：
4.9
作者：
Feng, Jie;Hwang, Ru;Peng, Gary
通讯作者：
Peng, Gary
影响因子：
4.5
作者：
Jiang RH;de Bruijn I;Haas BJ;Belmonte R;Löbach L;Christie J;van den Ackerveken G;Bottin A;Bulone V;Díaz-Moreno SM;Dumas B;Fan L;Gaulin E;Govers F;Grenville-Briggs LJ;Horner NR;Levin JZ;Mammella M;Meijer HJ;Morris P;Nusbaum C;Oome S;Phillips AJ;van Rooyen D;Rzeszutek E;Saraiva M;Secombes CJ;Seidl MF;Snel B;Stassen JH;Sykes S;Tripathy S;van den Berg H;Vega-Arreguin JC;Wawra S;Young SK;Zeng Q;Dieguez-Uribeondo J;Russ C;Tyler BM;van West P
通讯作者：
van West P
影响因子：
2.9
作者：
BJELLQVIST, B;HUGHES, GJ;HOCHSTRASSER, D
通讯作者：
HOCHSTRASSER, D
影响因子：
1.4
作者：
Fregeneda-Grandes, J. M.;Carbajal-Gonzalez, M. T.;Aller-Gancedo, J. M.
通讯作者：
Aller-Gancedo, J. M.
影响因子：
2.5
作者：
RICHARDS, RH;PICKERING, AD
通讯作者：
PICKERING, AD