Association of promoter methylation with histologic type and pleural indentation in non-small cell lung cancer (NSCLC).
Association of promoter methylation with histologic type and pleural indentation in non-small cell lung cancer (NSCLC).
复制标题
非小细胞肺癌 (NSCLC) 启动子甲基化与组织学类型和胸膜凹陷的关联
DOI：
10.1186/1746-1596-6-48
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发表时间：
2011-06-04
影响因子：
2.6
通讯作者：
Hou P
中科院分区：
文献类型：
--
作者：
Ji M;Zhang Y;Shi B;Hou P
背景肺癌是全世界死亡的主要原因。基因启动子甲基化是肿瘤相关基因的主要失活机制，其中一些基因可以作为肺癌早期诊断和预后评估的生物标志物。方法采用定量甲基化特异性PCR（Q-MSP）技术对96例临床特征良好的非小细胞肺癌（NSCLC）中6个基因的启动子甲基化进行检测。结果在NSCLC中发现了高频率的启动子甲基化。 6 个基因均具有 100% 的诊断特异性和 44.9% 至 84.1% 的高灵敏度。我们的数据还表明启动子甲基化与组织学类型密切相关。与腺癌 (ADC) 相比，大多数基因在鳞状细胞癌 (SCC) 中更频繁地甲基化。此外，启动子甲基化显着增加NSCLC胸膜凹陷的风险。结论我们的研究结果提供了多个基因在肺肿瘤发生过程中异常甲基化的证据，并证明启动子甲基化与NSCLC的临床病理特征密切相关。更重要的是，我们首次发现启动子甲基化可能是 NSCLC 患者胸膜凹陷的潜在增加危险因素。
BackgroundLung cancer is a major cause of death worldwide. Gene promoter methylation is a major inactivation mechanism of tumor-related genes, some of which can be served as a biomarker for early diagnosis and prognosis evaluation of lung cancer.MethodsWe determined the promoter methylation of 6 genes using quantitative methylation-specific PCR (Q-MSP) technique in 96 clinically well-characterized non-small cell lung cancer (NSCLC).ResultsHighly frequent promoter methylation was found in NSCLC. With 100% diagnostic specificity, high sensitivity, ranging from 44.9 to 84.1%, was found for each of the 6 genes. Our data also showed that promoter methylation was closely associated with histologic type. Most of genes were more frequently methylated in squamous cell carcinomas (SCC) compared to adenocarcinomas (ADC). Moreover, promoter methylation significantly increased the risk of pleural indentation in NSCLC.ConclusionOur findings provided evidences that multiple genes were aberrantly methylated in lung tumorigenesis, and demonstrated the promoter methylation was closely associated with clinicopathologic characteristics of NSCLC. More importantly, we first revealed promoter methylation may be served as a potentially increased risk factor for pleural indentation of NSCLC patients.
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影响因子：
254.7
作者：
Jemal, Ahmedin;Siegel, Rebecca;Thun, Michael J.
通讯作者：
Thun, Michael J.
DOI：
10.1158/1055-9965.epi-08-0192
发表时间：
2008-10
期刊：
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology
影响因子：
--
作者：
Brait M;Begum S;Carvalho AL;Dasgupta S;Vettore AL;Czerniak B;Caballero OL;Westra WH;Sidransky D;Hoque MO
通讯作者：
Hoque MO
DOI：
10.1111/j.1749-6632.2003.tb05973.x
发表时间：
2003-01-01
期刊：
EPIGENETICS IN CANCER PREVENTION: EARLY DETECTION AND RISK ASSESSMENT
影响因子：
--
作者：
Moore, LE;Huang, WY;Hayes, RB
通讯作者：
Hayes, RB
影响因子：
5.3
作者：
de Fraipont, F;Moro-Sibilot, D;Favrot, MC
通讯作者：
Favrot, MC
影响因子：
2.7
作者：
Christoffels, VM;Keijser, AGM;Moorman, AFM
通讯作者：
Moorman, AFM