In Vitro Selection of DNA Aptamers that Binds Geniposide.
In Vitro Selection of DNA Aptamers that Binds Geniposide.
复制标题
结合京尼平苷的 DNA 适体的体外选择
DOI：
10.3390/molecules22030383
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发表时间：
2017-02-28
期刊：
影响因子：
--
通讯作者：
Hua Q
中科院分区：
文献类型：
--
作者：
Zhang A;Chang D;Zhang Z;Li F;Li W;Wang X;Li Y;Hua Q
Geniposide is a key iridoid glycoside from Gardenia jasminoides fructus widely used in traditional Chinese herbal medicine. However, detection of this small molecule represents a significant challenge mostly due to the lack of specific molecular recognition elements. In this study, we have performed in vitro selection experiments to isolate DNA aptamers that can specifically bind geniposide. Using a stringent selection procedure, we have isolated DNA aptamers that can distinguish geniposide from genipin and glucose, two structural analogs of geniposide. Two top aptamers exhibit low micromolar binding affinity towards geniposide, but show significantly reduced affinity to genipin and glucose. These aptamers have the potential to be further developed into analytical tools for the detection of geniposide.
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影响因子：
3.2
作者：
Cho, Y. J.;Kim, S. Y.;Shin, H. -J.
通讯作者：
Shin, H. -J.
DOI：
10.1111/j.1440-1681.2006.04466.x
发表时间：
2006-10-01
影响因子：
2.9
作者：
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62.1
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通讯作者：
Lu, Yi
影响因子：
1.7
作者：
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通讯作者：
Zhang, Hai-Yan