C3HC4-type RING finger protein NbZFP1 is involved in growth and fruit development in Nicotiana benthamiana.
C3HC4-type RING finger protein NbZFP1 is involved in growth and fruit development in Nicotiana benthamiana.
复制标题
C3HC4型环指蛋白NbZFP1参与本塞姆氏烟草的生长和果实发育
DOI：
10.1371/journal.pone.0099352
复制
发表时间：
2014
期刊：
影响因子：
3.7
通讯作者：
Qiu D
中科院分区：
文献类型：
--
作者：
Wu W;Cheng Z;Liu M;Yang X;Qiu D
C3 HC 4型环指蛋白是植物界的一个大家族，在植物生命的各种生理过程中发挥着重要作用。本研究从本氏烟草（Nicotiana benthamiana）中克隆了一个C3 HC 4型锌指基因。序列分析表明，该基因编码一个24 kDa的蛋白，由191个氨基酸组成，含有一个典型的C3 HC 4型锌指结构域，命名为NbZFP 1。pGDG-NbZFP 1的瞬时表达表明NbZFP 1定位于叶绿体，尤其是叶气孔周围细胞的叶绿体中。病毒诱导的基因沉默（VIGS）分析表明，NbZFP 1的沉默阻碍了果实发育，虽然植物的高度是正常的。设计了一个过量表达的构建体，并将其转入本氏烟草中，PCR和Southern杂交结果表明，NbZFP 1基因已成功整合到本氏烟草基因组中。转基因株系表现出典型的紧凑性，节间短，茎粗壮。这是第一份描述烟草中C3 HC 4型RING指蛋白功能的报告。
C3HC4-type RING finger proteins constitute a large family in the plant kingdom and play important roles in various physiological processes of plant life. In this study, a C3HC4-type zinc finger gene was isolated from Nicotiana benthamiana. Sequence analysis indicated that the gene encodes a 24-kDa protein with 191 amino acids containing one typical C3HC4-type zinc finger domain; this gene was named NbZFP1. Transient expression of pGDG-NbZFP1 demonstrated that NbZFP1 was localized to the chloroplast, especially in the chloroplasts of cells surrounding leaf stomata. Virus-induced gene silencing (VIGS) analysis indicated that silencing of NbZFP1 hampered fruit development, although the height of the plants was normal. An overexpression construct was then designed and transferred into Nicotiana benthamiana, and PCR and Southern blot showed that the NbZFP1 gene was successfully integrated into the Nicotiana benthamiana genome. The transgenic lines showed typical compactness, with a short internode length and sturdy stems. This is the first report describing the function of a C3HC4-type RING finger protein in tobacco.
登录
查看更多内容
影响因子：
7.3
作者：
Kulye, Mahesh;Liu, Hua;Qiu, Dewen
通讯作者：
Qiu, Dewen
DOI：
10.1073/pnas.0500146102
发表时间：
2005-03-15
影响因子：
11.1
作者：
Levchenko, V;Konrad, KR;Hedrich, R
通讯作者：
Hedrich, R
影响因子：
7.2
作者：
Kobayashi, A;Sakamoto, A;Takatsuji, H
通讯作者：
Takatsuji, H
影响因子：
7.4
作者：
Dong, Yiyu;Burch-Smith, Tessa M.;Dinesh-Kumar, Savithramma P.
通讯作者：
Dinesh-Kumar, Savithramma P.
影响因子：
4.3
作者：
Liu, Kaimao;Wang, Lei;Chong, Kang
通讯作者：
Chong, Kang