Isolation, Characterization, and Application of Clostridium sporogenes F39 to Degrade Zearalenone under Anaerobic Conditions.
Isolation, Characterization, and Application of Clostridium sporogenes F39 to Degrade Zearalenone under Anaerobic Conditions.
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产孢梭菌 F39 的分离、表征及其在厌氧条件下降解玉米赤霉烯酮的应用
DOI：
10.3390/foods11091194
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发表时间：
2022-04-20
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影响因子：
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中科院分区：
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玉米赤霉烯酮（ZEN）由镰刀菌属（Fusarium spp.）广泛存在于发霉的小麦、玉米和其他谷物中。ZEN具有很强的毒性，并导致动物和人类的生殖和免疫障碍以及雌激素综合征。生物降解已被证明是控制ZEN危害的有效方法。在饲料中应用生物降解的一个有前途的方法是引入厌氧ZEN降解微生物，其可以在动物肠道的消化过程中发挥作用。本研究的目的是从厌氧环境中分离厌氧ZEN降解菌。从动物肠道内容物中分离到一株ZEN降解菌F39，48 h内ZEN降解率为87.35%，可生成微量的α-和β-玉米赤霉烯醇。通过形态学、生理生化特征及16 S rRNA和rpoB基因序列分析，将F39菌株鉴定为产孢梭菌（Clostridium sporogenes）。F39的最适生长温度为37 °C，最适pH为7.0，最适碳源为牛肉膏; ZEN的最适降解条件为35 °C，pH 7.0，GAM培养基。在1-15 mg/L的浓度范围内，F39对ZEN有较好的降解效果。降解ZEN的生物活性因子主要分布在细胞内。F39可以降解大部分存在的ZEN，但少量被分解为两种次级代谢产物，α-和β-玉米赤霉烯醇，降解产物的毒性降低。F39对小麦基饲料中ZEN的降解率为49%，比其他饲料中的ZEN降解率高，且降解效率与pH值有关。据我们所知，这是第一次报道生孢梭菌F39的能力，以保持生物降解潜力。
Zearalenone (ZEN) is produced by Fusarium spp. and is widely found in moldy wheat, corn, and other grains. ZEN has a strong toxicity and causes reproductive and immune disorders and estrogenic syndrome in animals and humans. Biodegradation has been demonstrated as an efficient way to control the hazardous effect of ZEN. A promising way to apply biodegradation in feed is to introduce anaerobic ZEN-degrading microorganisms, which can function during the digestion process in animal intestines. The aim of this study was to isolate anaerobic ZEN-degrading bacteria from anaerobic environments. A strain named F39 was isolated from animal intestinal contents and had a ZEN-degradation rate of 87.35% in 48 h to form trace amount of α- and β-zearalenol. Based on the morphological and physiological properties and phylogenetic analysis of 16S rRNA and rpoB gene sequences, F39 was identified as Clostridium sporogenes. The optimum temperature for the growth of F39 was 37 °C, the optimum pH was 7.0, and the most suitable carbon source was beef extract, while the optimal conditions for the degradation of ZEN were as follows: 35 °C, pH 7.0, and GAM medium. ZEN was degraded by F39 with a high efficiency in the concentration range of 1–15 mg/L. The bioactive factors responsible for ZEN degradation were mainly distributed intracellularly. F39 can degrade most of the ZEN present, but a small amount is broken down into two secondary metabolites, α- and β-zearalenol, and the toxicity of the degradation products is reduced. With an efficiency of 49%, F39 can more effectively degrade ZEN in wheat-based feedstuffs than in other feedstuff, and the degradation efficiency was pH related. To the best of our knowledge, this is the first report of Clostridium sporogenes F39’s ability to maintain the biodegradation potentials.
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影响因子：
2.1
作者：
Brodehl, Antje;Moeller, Anne;Maul, Ronald
通讯作者：
Maul, Ronald
影响因子：
9.9
作者：
Dakovic, Aleksandra;Matijasevic, Srdan;Clewett, Catherine F. M.
通讯作者：
Clewett, Catherine F. M.
影响因子：
0.8
作者：
Obremski, K.;Poniatowska-Broniek, G.
通讯作者：
Poniatowska-Broniek, G.
益生菌作为预防和治疗过敏的可能策略。叙事评论。
DOI：
10.3390/foods10040701
发表时间：
2021-03-25
期刊：
Foods (Basel, Switzerland)
影响因子：
--
作者：
Lopez-Santamarina A;Gonzalez EG;Lamas A;Mondragon ADC;Regal P;Miranda JM
通讯作者：
Miranda JM
影响因子：
10.7
作者：
Kumar, Sudhir;Stecher, Glen;Tamura, Koichiro
通讯作者：
Tamura, Koichiro