Embryonic ductal plate cells give rise to cholangiocytes, periportal hepatocytes, and adult liver progenitor cells.
Embryonic ductal plate cells give rise to cholangiocytes, periportal hepatocytes, and adult liver progenitor cells.
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DOI：
10.1053/j.gastro.2011.06.049
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发表时间：
2011-10
期刊：
影响因子：
29.4
通讯作者：
Lemaigre FP
中科院分区：
文献类型：
--
作者：
Carpentier R;Suñer RE;van Hul N;Kopp JL;Beaudry JB;Cordi S;Antoniou A;Raynaud P;Lepreux S;Jacquemin P;Leclercq IA;Sander M;Lemaigre FP
胚胎胆道前体细胞在门静脉周围形成一层称为胆管板的片状物，逐渐被改造形成肝内胆管。有限数量的导管板细胞参与导管的形成；那些不参与导管发育的细胞被认为与细胞凋亡有关。此外，表达sry相关的HMG盒子转录因子9 （SOX9）的细胞，包括胚胎导管板细胞，被认为可以持续地为肝脏提供肝细胞。我们研究了导管板在肝脏形态发生中的作用。用免疫染色法观察小鼠和人胎肝组织的凋亡和增殖。在基因标记后分析表达sox9的导管板细胞的出生后代，在导管板阶段，通过cre介导的ROSA26RYFP报告等位基因重组。通过插入细菌人工染色体的SOX9调控区诱导Cre的诱导表达。研究小鼠肝脏在正常条件下和在饮食诱导下的再生过程。导管板细胞未发生凋亡，增殖有限。它们在小叶间胆管、胆管和胆管内产生胆管细胞，以及门静脉周围肝细胞。在再生过程中出现的卵形细胞也来源于导管板。我们没有发现肝脏稳态需要来自表达sox9的祖细胞的持续供应。肝内胆管包括其最近端胆管段，在肝内胆管板上形成胆管细胞。它也产生门静脉周围肝细胞和成体肝祖细胞。
Embryonic biliary precursor cells form a periportal sheet called the ductal plate, which is progressively remodeled to generate intrahepatic bile ducts. A limited number of ductal plate cells participate in duct formation; those not involved in duct development are believed to involute by apoptosis. Moreover, cells that express the SRY-related HMG box transcription factor 9 (SOX9), which include the embryonic ductal plate cells, were proposed to continuously supply the liver with hepatic cells. We investigated the role of the ductal plate in hepatic morphogenesis. Apoptosis and proliferation were investigated by immunostaining of mouse and human fetal liver tissue. The post-natal progeny of SOX9-expressing ductal plate cells was analysed after genetic labeling, at the ductal plate stage, by Cre-mediated recombination of a ROSA26RYFP reporter allele. Inducible Cre expression was induced by SOX9 regulatory regions, inserted in a bacterial artificial chromosome. Livers were studied from mice under normal conditions and during diet-induced regeneration. Ductal plate cells did not undergo apoptosis and showed limited proliferation. They generated cholangiocytes lining interlobular bile ducts, bile ductules and canals of Hering, as well as periportal hepatocytes. Oval cells that appeared during regeneration also derived from the ductal plate. We did not find that liver homeostasis required a continuous supply of cells from SOX9-expressing progenitors. The ductal plate gives rise to cholangiocytes lining the intrahepatic bile ducts, including its most proximal segments. It also generates periportal hepatocytes and adult hepatic progenitor cells.
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DOI：
10.1002/ar.20710
发表时间：
2008-06-01
影响因子：
2
作者：
Roskams, T.;Desmet, V.
通讯作者：
Desmet, V.
影响因子：
13.5
作者：
Paku, S;Dezsö, K;Nagy, P
通讯作者：
Nagy, P
影响因子：
--
作者：
Srinivas S;Watanabe T;Lin CS;William CM;Tanabe Y;Jessell TM;Costantini F
通讯作者：
Costantini F
影响因子：
3.2
作者：
Pirici, Daniel;Mogoanta, Laurentiu;Stanescu, Radu
通讯作者：
Stanescu, Radu
影响因子：
13.5
作者：
Zhang, Lili;Theise, Neil;Reid, Lola M.
通讯作者：
Reid, Lola M.