A specialized spinal circuit for command amplification and directionality during escape behavior.
A specialized spinal circuit for command amplification and directionality during escape behavior.
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一种专门的脊髓回路，用于在逃跑行为期间放大命令和方向性
DOI：
10.1073/pnas.2106785118
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发表时间：
2021-10-19
影响因子：
11.1
通讯作者：
Song J
中科院分区：
文献类型：
--
作者：
Guan NN;Xu L;Zhang T;Huang CX;Wang Z;Dahlberg E;Wang H;Wang F;Pallucchi I;Hua Y;El Manira A;Song J
意义我们经常面临向左或向右移动的选择;从生物学和临床角度来看，了解大脑如何解决行动方向的选择是非常有趣的。在脊椎动物中，动作选择通常被认为是高级认知处理的领域。然而，通过结合电生理学，连续块面电子显微镜，和行为分析在斑马鱼，我们已经揭示了一个关键的作用，以及一个专门的脊髓电路依赖于强大的轴-轴突触连接的全功能连接体。这包括识别一类胆碱能V2 a中间神经元，并确定它们作为一个分段重复的枢纽，接收和放大来自大脑的逃避命令，以确保适当的逃避方向性。在脊椎动物中，行动选择通常涉及更高的认知，这需要一个评估过程。然而，紧急任务，如防御性逃逸，需要立即实施逃逸轨迹的方向性，需要本地电路。在这里，我们揭示了一个专门的脊髓回路执行逃生方向在成年斑马鱼。这个回路的一个中心组成部分是一类独特的分段重复的胆碱能V2 a中间神经元表达的转录因子Chx 10。这些中间神经元放大脑干发起的逃避命令，并通过前馈电路迅速将兴奋传递到所有快运动神经元和连合中间神经元，以指导逃避动作。这个回路中的信息传递依赖于快速可靠的轴-轴突触连接，绕过索马和树突。胆碱能V2 a中间神经元的单侧消融消除了逃逸命令传播。因此，在脊椎动物中，局部脊髓回路可以实现对生存至关重要的紧急运动动作的方向性。
Significance We are constantly faced with a choice moving to the left or right; understanding how the brain solves the selection of action direction is of tremendous interest both from biological and clinical perspectives. In vertebrates, action selection is often considered to be the realm of higher cognitive processing. However, by combining electrophysiology, serial block-face electron microscopy, and behavioral analyses in zebrafish, we have revealed a pivotal role, as well as the full functional connectome of a specialized spinal circuit relying on strong axo-axonic synaptic connections. This includes identifying a class of cholinergic V2a interneurons and establishing that they act as a segmentally repeating hub that receives and amplifies escape commands from the brain to ensure the appropriate escape directionality. In vertebrates, action selection often involves higher cognition entailing an evaluative process. However, urgent tasks, such as defensive escape, require an immediate implementation of the directionality of escape trajectory, necessitating local circuits. Here we reveal a specialized spinal circuit for the execution of escape direction in adult zebrafish. A central component of this circuit is a unique class of segmentally repeating cholinergic V2a interneurons expressing the transcription factor Chx10. These interneurons amplify brainstem-initiated escape commands and rapidly deliver the excitation via a feedforward circuit to all fast motor neurons and commissural interneurons to direct the escape maneuver. The information transfer within this circuit relies on fast and reliable axo-axonic synaptic connections, bypassing soma and dendrites. Unilateral ablation of cholinergic V2a interneurons eliminated escape command propagation. Thus, in vertebrates, local spinal circuits can implement directionality of urgent motor actions vital for survival.
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影响因子：
25
作者：
Cregg JM;Leiras R;Montalant A;Wanken P;Wickersham IR;Kiehn O
通讯作者：
Kiehn O
影响因子：
25
作者：
Gabriel, Jens Peter;Ausborn, Jessica;El Manira, Abdeljabbar
通讯作者：
El Manira, Abdeljabbar
DOI：
10.1016/j.cub.2017.08.012
发表时间：
2017-09-25
期刊：
Current biology : CB
影响因子：
--
作者：
Bhattacharyya K;McLean DL;MacIver MA
通讯作者：
MacIver MA
影响因子：
7.2
作者：
Graybiel, Ann M.;Grafton, Scott T.
通讯作者：
Grafton, Scott T.
DOI：
10.1073/pnas.1216256110
发表时间：
2012-12-26
影响因子：
11.1
作者：
Ausborn, Jessica;Mahmood, Riyadh;El Manira, Abdeljabbar
通讯作者：
El Manira, Abdeljabbar