The molecular architecture of Drosophila melanogaster defense against Beauveria bassiana explored through evolve and resequence and quantitative trait locus mapping.
The molecular architecture of Drosophila melanogaster defense against Beauveria bassiana explored through evolve and resequence and quantitative trait locus mapping.
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DOI：
10.1093/g3journal/jkab324
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发表时间：
2021-12-08
期刊：
影响因子：
--
通讯作者：
Lazzaro BP
中科院分区：
文献类型：
--
作者：
Shahrestani P;King E;Ramezan R;Phillips M;Riddle M;Thornburg M;Greenspan Z;Estrella Y;Garcia K;Chowdhury P;Malarat G;Zhu M;Rottshaefer SM;Wraight S;Griggs M;Vandenberg J;Long AD;Clark AG;Lazzaro BP
人们对自然群体中抗真菌免疫的遗传结构知之甚少。使用两种群体遗传方法、数量性状位点 (QTL) 作图以及进化和重测序 (E&R)，我们探索了黑腹果蝇针对真菌白僵菌感染的免疫防御。在用于我们的 QTL 定位的果蝇合成种群资源的重组自交系和我们的 E&R 研究中使用的合成远交种群中，免疫防御都存在很大差异。在 E&R 研究中，仅 10 代的感染存活率就显着提高，并且在另外 9 代中继续增加，揭示了与未感染寿命的权衡。为增强对白僵菌的防御而选择的群体进化出对第二种不同的白僵菌菌株的交叉抗性，但对细菌病原体没有交叉抗性。 QTL作图研究表明，防御中的性二态性取决于宿主基因型，而E&R研究表明，性二态性还取决于宿主所接触的特定病原体。 QTL 作图和 E&R 实验都生成了潜在因果候选基因的列表，尽管这些列表不重叠。
Little is known about the genetic architecture of antifungal immunity in natural populations. Using two population genetic approaches, quantitative trait locus (QTL) mapping and evolve and resequence (E&R), we explored D. melanogaster immune defense against infection with the fungus Beauveria bassiana. The immune defense was highly variable both in the recombinant inbred lines from the Drosophila Synthetic Population Resource used for our QTL mapping and in the synthetic outbred populations used in our E&R study. Survivorship of infection improved dramatically over just 10 generations in the E&R study, and continued to increase for an additional nine generations, revealing a trade-off with uninfected longevity. Populations selected for increased defense against B. bassiana evolved cross resistance to a second, distinct B. bassiana strain but not to bacterial pathogens. The QTL mapping study revealed that sexual dimorphism in defense depends on host genotype, and the E&R study indicated that sexual dimorphism also depends on the specific pathogen to which the host is exposed. Both the QTL mapping and E&R experiments generated lists of potentially causal candidate genes, although these lists were nonoverlapping.
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DOI：
10.1006/bbrc.2001.5067
发表时间：
2001-06-22
影响因子：
3.1
作者：
Ekengren, S;Hultmark, D
通讯作者：
Hultmark, D
DOI：
10.1111/j.1095-8312.2003.00271.x
发表时间：
2003-12-01
影响因子：
1.9
作者：
Bochdanovits, Z;De Jong, G
通讯作者：
De Jong, G
影响因子：
3.3
作者：
Faria, Vitor G.;Martins, Nelson E.;Magalhaes, Sara
通讯作者：
Magalhaes, Sara
影响因子：
1.2
作者：
Early, Angela M.;Clark, Andrew G.
通讯作者：
Clark, Andrew G.
影响因子：
2.1
作者：
Bubliy, OA;Loeschcke, V
通讯作者：
Loeschcke, V