Cellular immune response to parasitization in Drosophila requires the EBF orthologue collier.
Cellular immune response to parasitization in Drosophila requires the EBF orthologue collier.
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DOI：
10.1371/journal.pbio.0020196
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发表时间：
2004-08
期刊：
影响因子：
9.8
通讯作者：
Meister M
中科院分区：
文献类型：
--
作者：
Crozatier M;Ubeda JM;Vincent A;Meister M
果蝇的免疫反应涉及三种类型的血细胞。一种细胞类型，板层细胞，只有在特定条件下才会被诱导分化，比如被黄蜂寄生。在这里，我们研究了层状细胞规范的机制。我们首先发现，编码早期b细胞因子（EBF）的果蝇同源基因collier （col）在幼虫造血器官淋巴腺的个体发育过程中表达得非常早。在这个器官中，Col的表达预示着一个特定的后部区域最近被认为是信号传导中心，即后部信号传导中心（PSC）。寄生的col突变体幼虫完全缺乏层板细胞，揭示了这种细胞类型对col活性的关键要求。在野生型幼虫中，尽管有大量板层细胞产生，但Col的表达仍然局限于PSC。因此，我们提出，Col赋予PSC细胞传递指导性信号的能力，使造血前体在寄生反应中朝向板层细胞的命运。考虑到EBF在淋巴生成中的作用，这些发现表明果蝇和脊椎动物之间的细胞免疫有新的相似之处。进一步研究其他门中Col/EBF的表达和功能将为淋巴样细胞的进化起源提供新的见解。板层细胞在黄蜂寄生等条件下被诱导分化，并在本研究中显示需要collier，这是脊椎动物早期b细胞因子的同源物
Drosophila immune response involves three types of hemocytes (‘blood cells’). One cell type, the lamellocyte, is induced to differentiate only under particular conditions, such as parasitization by wasps. Here, we have investigated the mechanisms underlying the specification of lamellocytes. We first show that collier (col), the Drosophila orthologue of the vertebrate gene encoding early B-cell factor (EBF), is expressed very early during ontogeny of the lymph gland, the larval hematopoietic organ. In this organ, Col expression prefigures a specific posterior region recently proposed to act as a signalling centre, the posterior signalling centre (PSC). The complete lack of lamellocytes in parasitized col mutant larvae revealed the critical requirement for Col activity in specification of this cell type. In wild-type larvae, Col expression remains restricted to the PSC following parasitization, despite the massive production of lamellocytes. We therefore propose that Col endows PSC cells with the capacity to relay an instructive signal that orients hematopoietic precursors towards the lamellocyte fate in response to parasitization. Considered together with the role of EBF in lymphopoiesis, these findings suggest new parallels in cellular immunity between Drosophila and vertebrates. Further investigations on Col/EBF expression and function in other phyla should provide fresh insight into the evolutionary origin of lymphoid cells. The lamellocyte is induced to differentiate under conditions such as parasitization by wasps and is shown in this study to require collier, the orthologue of the vertebrate early B-cell factor
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影响因子：
11.4
作者：
LUO, H;HANRATTY, WP;DEAROLF, CR
通讯作者：
DEAROLF, CR
影响因子：
9.2
作者：
Duvic, B;Hoffmann, JA;Royet, J
通讯作者：
Royet, J
影响因子：
4.6
作者：
Holz, A;Bossinger, B;Klapper, R
通讯作者：
Klapper, R
DOI：
10.1073/pnas.212527499
发表时间：
2002-10-29
影响因子：
11.1
作者：
Mayer, WE;Uinuk-ool, T;Cooper, MD
通讯作者：
Cooper, MD
影响因子：
11.4
作者：
HARRISON, DA;BINARI, R;PERRIMON, N
通讯作者：
PERRIMON, N