Solid freeform fabrication of designer scaffolds of hyaluronic acid for nerve tissue engineering.
Solid freeform fabrication of designer scaffolds of hyaluronic acid for nerve tissue engineering.
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DOI：
10.1007/s10544-011-9568-9
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发表时间：
2011-12
影响因子：
2.8
通讯作者：
Schmidt CE
中科院分区：
文献类型：
--
作者：
Suri S;Han LH;Zhang W;Singh A;Chen S;Schmidt CE
组织工程和再生医学领域将极大地受益于三维支架的发展，其具有复制天然组织的几何形状和化学组成的限定的微观和宏观结构。目前的报告描述了一种自由成型的制造技术，允许神经再生支架的发展与精确的工程结构，模仿天然神经，使用天然细胞外基质成分透明质酸（HA）。为了证明制造系统的灵活性，以逐层的方式制造具有不同孔形状、尺寸和可控降解性的不同几何形状的支架。为了促进细胞粘附，用层粘连蛋白共价官能化支架。这种方法提供了巨大的时空灵活性，以创建建筑上复杂的结构，例如具有分支管的支架，以模拟神经丛处的分支神经。我们进一步证明了在微制造的神经导管内创建双向梯度的能力。我们相信，结合HA的生物学特性与精确的三维微结构可以为开发广泛的生物人工器官提供一个有用的平台。
The field of tissue engineering and regenerative medicine will tremendously benefit from the development of three dimensional scaffolds with defined micro- and macro-architecture that replicate the geometry and chemical composition of native tissues. The current report describes a freeform fabrication technique that permits the development of nerve regeneration scaffolds with precisely engineered architecture that mimics that of native nerve, using the native extracellular matrix component hyaluronic acid (HA). To demonstrate the flexibility of the fabrication system, scaffolds exhibiting different geometries with varying pore shapes, sizes and controlled degradability were fabricated in a layer-by-layer fashion. To promote cell adhesion, scaffolds were covalently functionalized with laminin. This approach offers tremendous spatio-temporal flexibility to create architecturally complex structures such as scaffolds with branched tubes to mimic branched nerves at a plexus. We further demonstrate the ability to create bidirectional gradients within the microfabricated nerve conduits. We believe that combining the biological properties of HA with precise three dimensional micro-architecture could offer a useful platform for the development of a wide range of bioartificial organs.
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