Induction of epithelio-mesenchymal transformation of quail embryonic neural cells by inhibition of atypical protein kinase-C
Induction of epithelio-mesenchymal transformation of quail embryonic neural cells by inhibition of atypical protein kinase-C
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抑制非典型蛋白激酶-C诱导鹌鹑胚胎神经细胞上皮间质转化
DOI：
--
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发表时间：
1999
影响因子：
3.6
通讯作者：
D. Newgreen
中科院分区：
文献类型：
--
作者：
J. Minichiello;A. Ben;C. Hearn;B. Needham;D. Newgreen
在体外培养的胚胎神经上皮中，蛋白激酶-c抑制剂可诱导细胞间质转化，其中包括细胞粘附分子和肌动蛋白骨架的重组。一种非抑制性类似物，BIM V和其他激酶的有效抑制剂没有活性。这表明了c激酶的核心作用，尽管强大的特异性c激酶抑制剂BIM I和Ro 31-8220的活性低于预期。几种激酶的共抑制不太可能解释这一点，因为当这些激酶与其他激酶的强效抑制剂联合使用时，不会发生增强。BIM I和Ro 31-8220仅强烈抑制常规钙调节的c激酶；这一点以及TMB-8缺乏抑制钙释放的作用，表明涉及新的和/或非典型亚型。常规和新型c -激酶的各种增强剂和激活剂单独作用不明显，不能降低staurosporine的作用，提示非典型c -激酶是关键。在Western blots上检测了E2胚胎神经组织中c -激酶亚型的存在，揭示了非典型亚型ι（或λ）和ζ以及α， γ， κ和μ亚型的免疫反应性。胚胎切片的免疫荧光定位显示，常规和新型异构体广泛分布，但只有非典型异构体λ和ζ在神经和其他上皮的顶端边缘富集；它们与细胞粘附分子n -钙粘蛋白和f -肌动蛋白重叠。因此，体外胚胎神经上皮上皮间质转化是通过抑制蛋白激酶活性诱导的，可能是通过非典型蛋白激酶c；非典型蛋白激酶- c亚型存在于组织的适当发育阶段和亚细胞部位的细胞能够上皮-间质转化。
Abstract Epithelio-mesenchymal transition, which involves the re-organisation of cell-cell adhesion molecules and the actin cytoskeleton, can be induced in embryonic neural epithelium in vitro by protein kinase-C inhibitors. A non-inhibitory analogue, BIM V, and potent inhibitors of other kinases are not active. This suggests a central role for C-kinases, although the powerful specific C-kinase inhibitors BIM I and Ro 31–8220 show lower than expected activity. Co-inhibition by several kinases is unlikely to account for this, since no potentiation occurs when these are combined with potent inhibitors of other kinases. BIM I and Ro 31–8220 strongly inhibit only conventional calcium-regulated C-kinases; this and the lack of effect of TMB-8, which inhibits calcium release, suggests that novel and/or atypical isoforms are involved. Various potentiators and activators of conventional and novel C-kinases have no obvious effect alone and fail to reduce the effect of staurosporine, suggesting that atypical C-kinases are critical. The presence of C-kinase isoforms in the E2 embryonic neural tissues has been probed on Western blots, revealing immunoreactivity for the atypical isoforms ι (or λ) and ζ and the α, γ, ɛ and μ isoforms. Immunofluorecent localisation on sections of embryos has shown the widespread distribution of conventional and novel isoforms but only the atypical isoforms λ and ζ are enriched at the apical margins of the neural and other epithelia; they overlap with the cell-cell adhesion molecule N-cadherin and with F-actin. Thus, epithelio-mesenchymal transition in the embryonic neural epithelium in vitro is induced by inhibiting protein kinase activity, probably via an atypical protein kinase-C; atypical protein kinase-C isoforms are present in the tissue at the appropriate developmental stage and subcellular site in cells capable of epithelio-mesenchymal transition.
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DOI：
10.1159/000147748
发表时间：
1995
期刊：
Acta anatomica
影响因子：
--
作者：
E. Hay
通讯作者：
E. Hay
影响因子：
7.5
作者：
MIEKKA, SI;INGHAM, KC;MENACHE, D
通讯作者：
MENACHE, D
鸡胚胎背神经管和神经嵴消融后体节中细胞趋化素的表达。
DOI：
10.1073/pnas.88.15.6398
发表时间：
1991
影响因子：
11.1
作者：
Tan,SS;Prieto,AL;Newgreen,DF;Crossin,KL;Edelman,GM
通讯作者：
Edelman,GM
影响因子：
56.9
作者：
LUNA, EJ;HITT, AL
通讯作者：
HITT, AL