CMV Infection Is Directly Related to the Inflammatory Status in Chronic Heart Failure Patients.
CMV Infection Is Directly Related to the Inflammatory Status in Chronic Heart Failure Patients.
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CMV感染与慢性心力衰竭患者的炎症状态直接相关。
DOI：
10.3389/fimmu.2021.687582
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发表时间：
2021
影响因子：
7.3
通讯作者：
Moro-García MA
中科院分区：
文献类型：
--
作者：
García-Torre A;Bueno-García E;López-Martínez R;Rioseras B;Díaz-Molina B;Lambert JL;Quirós C;Alonso-Álvarez S;Alonso-Arias R;Moro-García MA
高水平的炎症在慢性心力衰竭（CHF）中起着重要作用。CHF患者全身循环的促炎细胞因子水平升高，主要是TNF和IL-6。然而，几乎没有研究表明这些水平与CHF患者的功能状态有关，更不用说与他们的CMV血清状态有关。在这项研究中，分析了CHF患者（n=40;年龄=54.9 ± 6.3;纽约心脏协会心功能分级（NYHA，I-III））和健康对照组（n=40;年龄=53.5 ± 7.1）。通过Luminex® xMap Technology测量9种促炎和抗炎细胞因子的血清浓度，并通过TaqMan™ Array定量CD 4 + T淋巴细胞中一些免疫分子的mRNA表达的基础水平。还测量了响应于抗CD 3和LPS的培养上清液中这些细胞因子的浓度。测定CD 28无效T细胞的百分比以及抗CMV的抗体滴度。我们发现，与健康对照组相比，CHF血清中研究的所有细胞因子浓度更高，CHF患者的功能状态与炎性细胞因子水平之间存在直接相关性。此外，最高的细胞因子浓度被发现在具有较高浓度的缺乏CD 28分子的淋巴细胞的患者中。CMV+患者的细胞因子产生要高得多，并且这些细胞因子的产生主要在CMV+患者的T淋巴细胞中发现，以响应抗CD 3。抗CMV抗体水平与细胞因子水平呈正相关。参与Th 1应答的主要分子以及与CD 4 + CD 28无效亚群相关的分子的特异性mRNA的基线表达在CMV+患者中更高。CHF CMV+患者的细胞因子浓度较高，这些浓度与抗CMV抗体的产生有关。这些高水平的细胞因子也与更分化的CD 28 null淋巴细胞群相关。所有这些，加上病理本身的动态，使CMV+患者呈现更差的功能状态，并可能出现更差的病理演变。
High levels of inflammation play an important role in chronic heart failure (CHF). Patients with CHF have elevated levels of pro-inflammatory cytokines circulating systemically, mainly TNF and IL-6. However, there are almost no studies that relate these levels to the functional status of patients in CHF, much less to their CMV serostatus. In this study, patients with CHF (n=40; age=54.9 ± 6.3; New York Heart Association functional classification (NYHA, I-III) and healthy controls (n=40; age=53.5 ± 7.1) were analyzed. The serum concentrations of nine pro- and anti-inflammatory cytokines were measured by Luminex® xMap Technology and the basal level of mRNA expression of some immune molecules was quantified by TaqMan™ Array in CD4+ T-lymphocytes. The concentration of these cytokines in culture supernatants in response to anti-CD3 and LPS was also measured. The percentage of CD28null T-cells was determined, as well as the antibody titer against CMV. We found a higher concentration of all cytokines studied in CHF serum compared to healthy controls, as well as a direct correlation between functional status in CHF patients and levels of inflammatory cytokines. Moreover, the highest cytokine concentrations were found in patients with higher concentrations of lymphocytes lacking CD28 molecule. The cytokine production was much higher in CMV+ patients, and the production of these cytokines was found mainly in the T-lymphocytes of CMV+ patients in response to anti-CD3. Anti-CMV antibody levels were positively correlated with cytokine levels. The baseline expression of specific mRNA of the main molecules involved in the Th1 response, as well as molecules related to the CD4+CD28 null subset was higher in CMV+ patients. The cytokine concentrations are higher in CHF CMV+ patients and these concentrations are related to the production of antibodies against CMV. These high levels of cytokines are also associated with the more differentiated CD28null lymphocyte populations. All this, together with the dynamics of the pathology itself, makes CMV+ patients present a worse functional status and possibly a worse evolution of the pathology.
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影响因子：
3.4
作者：
Hartupee, Justin;Mann, Douglas L.
通讯作者：
Mann, Douglas L.
影响因子：
4.2
作者：
Isnard S;Fombuena B;Sadouni M;Lin J;Richard C;Routy B;Ouyang J;Ramendra R;Peng X;Zhang Y;Finkelman M;Tremblay-Sher D;Tremblay C;Chartrand-Lefebvre C;Durand M;Routy JP
通讯作者：
Routy JP
影响因子：
3.5
作者：
Antonio Moro-Garcia, Marco;Echeverria, Ainara;Alonso-Arias, Rebeca
通讯作者：
Alonso-Arias, Rebeca
影响因子：
7.8
作者：
Alonso-Arias, Rebeca;Moro-Garcia, Marco A.;Lopez-Larrea, Carlos
通讯作者：
Lopez-Larrea, Carlos
影响因子：
11.3
作者：
Goodrum F
通讯作者：
Goodrum F