Latex-specific transcriptome analysis reveals mechanisms for latex metabolism and natural rubber biosynthesis in laticifers of Hevea brasiliensis
Latex-specific transcriptome analysis reveals mechanisms for latex metabolism and natural rubber biosynthesis in laticifers of Hevea brasiliensis
复制标题
乳胶特异性转录组分析揭示了巴西橡胶树乳胶乳胶代谢和天然橡胶生物合成的机制
DOI：
10.1016/j.indcrop.2021.113835
复制
发表时间：
2021-11
影响因子：
5.9
通讯作者：
Xiao XH
中科院分区：
文献类型：
--
作者：
Long XY;Fang YJ;Qin YX;Yang JH;Xiao XH
产于准橡胶树（巴西橡胶树）乳汁管中的天然橡胶是一种具有商业和战略意义的宝贵生物聚合物。虽然橡胶生物合成的基本途径已经建立，但通过研究乳汁虫特异性基因的表达，可以进一步了解其调控机制。本文研究了橡胶树五种不同组织的基因表达模式，以探索差异表达基因（DEGs）。乳汁管中4122个高表达基因和6485个低表达基因在橡胶生物合成相关的多种代谢途径中富集表达。研究结果表明，天然橡胶的主要前体异戊烯基焦磷酸（IPP）在橡胶生物合成过程中主要通过甲羟戊酸（MVA）途径而不是2- c -甲基- d -erythritol-4-磷酸（MEP）途径提供。乳汁管中的泛素-蛋白酶体系统（UBS）异常活跃，可能是橡胶生物合成过程中重要的代谢控制机制。研究发现乳胶特异性转录因子（latex specific transcription factors, TFs）参与了7种植物激素信号转导，提示茉莉酸（jasmonate， JA）信号在橡胶生物合成过程中具有积极的正向调节作用。
Natural rubber, produced in the laticifers of the para rubber tree (Hevea brasiliensis), is a valuable biopolymer of commercial and strategic importance. While the basic pathway of rubber biosynthesis has been established, further understanding of its regulatory mechanisms might be obtained by studying laticifer-specific gene expression. Here, gene expression patterns was investigated in five different tissues of the rubber tree to explore differentially expressed genes (DEGs). The expression of 4122 high expression genes and 6485 low expression genes in the laticifer was enriched in various metabolic pathways related to rubber biosynthesis. The study results suggest that isopentenyl pyrophosphate (IPP), a principal precursor of natural rubber, is supplied mainly via the mevalonate (MVA) pathway in rubber biosynthesis, rather than the 2-C-methyl-D-erythritol-4-phosphate (MEP) pathway. The ubiquitin-proteasome system (UBS) that is surprisingly active in the laticifer might serve as an important metabolic control mechanism in rubber biosynthesis. The involvement of latex specific transcription factors (TFs) was identified in seven plant hormone signal transductions, suggesting that jasmonate (JA) signaling is active in the positive regulation of rubber biosynthesis.
登录
查看更多内容
影响因子：
2.9
作者：
Zhili Zhang;Jiahong Zhu
通讯作者：
Jiahong Zhu
影响因子：
4.6
作者：
Lau NS;Makita Y;Kawashima M;Taylor TD;Kondo S;Othman AS;Shu-Chien AC;Matsui M
通讯作者：
Matsui M
影响因子：
4.4
作者：
Rahman AY;Usharraj AO;Misra BB;Thottathil GP;Jayasekaran K;Feng Y;Hou S;Ong SY;Ng FL;Lee LS;Tan HS;Sakaff MK;Teh BS;Khoo BF;Badai SS;Aziz NA;Yuryev A;Knudsen B;Dionne-Laporte A;Mchunu NP;Yu Q;Langston BJ;Freitas TA;Young AG;Chen R;Wang L;Najimudin N;Saito JA;Alam M
通讯作者：
Alam M
影响因子：
7.8
作者：
Chang, Wei-chen;Song, Heng;Liu, Hung-wen;Lu, Pinghua
通讯作者：
Lu, Pinghua
Pará橡胶树基因组和多转录组数据库的构建加速了橡胶研究。
影响因子：
4.4
作者：
Makita Y;Kawashima M;Lau NS;Othman AS;Matsui M
通讯作者：
Matsui M