Comparison of therapeutic effects between pulsed and continuous wave 810-nm wavelength laser irradiation for traumatic brain injury in mice.
Comparison of therapeutic effects between pulsed and continuous wave 810-nm wavelength laser irradiation for traumatic brain injury in mice.
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DOI：
10.1371/journal.pone.0026212
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Hamblin MR
中科院分区：
文献类型：
--
作者：
Ando T;Xuan W;Xu T;Dai T;Sharma SK;Kharkwal GB;Huang YY;Wu Q;Whalen MJ;Sato S;Obara M;Hamblin MR
使用近红外光的经颅低水平激光治疗（LLLT）可以有效地穿透头皮和颅骨，并可以对创伤性脑损伤（TBI）进行非侵入性治疗。在本研究中，我们比较了使用810 nm波长的激光在连续和脉冲波模式在TBI小鼠模型的治疗效果。TBI由受控皮质撞击装置诱导，TBI后4小时，1组接受假治疗，3组接受经颅LLLT单次暴露，连续波或10 Hz或100 Hz脉冲，占空比为50%。810 nm Ga-Al-As二极管激光器以50 mW/cm 2的功率密度将直径为1 cm的光斑传递到受伤的头部上，持续12分钟，能量密度为36 J/cm 2。测量神经系统严重程度评分（NSS）和体重长达4周。在TBI后2、15和28天处死小鼠，并对病变大小进行组织学分析。在激光照射后立即测定脑组织中ATP产生的量。采用悬尾法和强迫游泳法检测LLLT对TBI后第1天和第4周小鼠心理状态的影响。通过NSS和体重的改善判断，以10 Hz脉冲的810 nm激光最有效，尽管其他激光方案也有效。10 Hz脉冲激光照射组小鼠脑损伤体积在伤后15 d和4周均显著低于对照组。此外，我们发现LLLT在4周时具有抗抑郁作用，如强迫游泳和悬尾试验所示。810 nm激光治疗TBI的LLLT治疗效果在10 Hz脉冲频率下比CW和100 Hz更有效。这一发现可能为LLLT的生物学机制提供新的见解。
Transcranial low-level laser therapy (LLLT) using near-infrared light can efficiently penetrate through the scalp and skull and could allow non-invasive treatment for traumatic brain injury (TBI). In the present study, we compared the therapeutic effect using 810-nm wavelength laser light in continuous and pulsed wave modes in a mouse model of TBI. TBI was induced by a controlled cortical-impact device and 4-hours post-TBI 1-group received a sham treatment and 3-groups received a single exposure to transcranial LLLT, either continuous wave or pulsed at 10-Hz or 100-Hz with a 50% duty cycle. An 810-nm Ga-Al-As diode laser delivered a spot with diameter of 1-cm onto the injured head with a power density of 50-mW/cm2 for 12-minutes giving a fluence of 36-J/cm2. Neurological severity score (NSS) and body weight were measured up to 4 weeks. Mice were sacrificed at 2, 15 and 28 days post-TBI and the lesion size was histologically analyzed. The quantity of ATP production in the brain tissue was determined immediately after laser irradiation. We examined the role of LLLT on the psychological state of the mice at 1 day and 4 weeks after TBI using tail suspension test and forced swim test. The 810-nm laser pulsed at 10-Hz was the most effective judged by improvement in NSS and body weight although the other laser regimens were also effective. The brain lesion volume of mice treated with 10-Hz pulsed-laser irradiation was significantly lower than control group at 15-days and 4-weeks post-TBI. Moreover, we found an antidepressant effect of LLLT at 4-weeks as shown by forced swim and tail suspension tests. The therapeutic effect of LLLT for TBI with an 810-nm laser was more effective at 10-Hz pulse frequency than at CW and 100-Hz. This finding may provide a new insight into biological mechanisms of LLLT.
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影响因子：
2.4
作者：
Brondon, Philip;Stadler, Istvan;Lanzafame, Raymond J.
通讯作者：
Lanzafame, Raymond J.
影响因子：
4.2
作者：
Dixon, CE;Kochanek, PM;DeKosky, ST
通讯作者：
DeKosky, ST
影响因子：
6.5
作者：
Hu, Wan-Ping;Wang, Jeh-Jeng;Yu, Hsin-Su
通讯作者：
Yu, Hsin-Su
影响因子：
2.4
作者：
Castro-e-Silva, O;Zucoloto, S;Bagnato, VS
通讯作者：
Bagnato, VS
DOI：
10.1097/ta.0b013e3181493080
发表时间：
2007-12-01
影响因子：
--
作者：
Faul, Mark;Wald, Marlena M.;Sattin, Richard W.
通讯作者：
Sattin, Richard W.