The posthatch prophylactic use of ceftiofur affects the cecal microbiota similar to the dietary sanguinarine supplementation in broilers.
The posthatch prophylactic use of ceftiofur affects the cecal microbiota similar to the dietary sanguinarine supplementation in broilers.
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DOI：
10.1016/j.psj.2020.06.078
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发表时间：
2020-11
期刊：
影响因子：
4.4
通讯作者：
Oliveira CJB
中科院分区：
文献类型：
--
作者：
Lemos MPL;Saraiva MMS;Leite EL;Silva NMV;Vasconcelos PC;Giachetto PF;Freitas Neto OC;Givisiez PEN;Gebreyes WA;Oliveira CJB
对新孵化的雏鸡预防性给予头孢噻呋是全球一些孵化场的常见做法，以减轻肠杆菌科引起的早期胃肠道感染。尽管肠道微生物组对肉鸡的健康起着至关重要的作用，但关于微生物组成如何受到这种程序的影响的信息仍然有限。我们研究了孵化后预防性应用头孢噻呋对饲喂常规或添加血根碱饲料的14日龄肉鸡盲肠微生物群的影响。从盲肠内容物中提取DNA样品，扩增微生物16 S rRNA基因的V3-V4区域，并在高通量测序平台（Illumina MiSeq）中测序。在下游生物信息学和统计分析后，我们的结果表明，头孢噻呋和血根碱处理同样增加了拟杆菌门和拟杆菌属和巨单胞菌属的比例，而减少了鸟类盲肠中厚壁菌门和毛螺菌科的相对丰度。这种变化可能与有利于短链脂肪酸产生的碳水化合物发酵过程的增加有关。功能预测结果也证实了这一点，表明接受头孢噻呋的肉鸡中与消化率相关的一些代谢途径增加。考虑到动物生产系统中的抗菌剂管理是非常需要的，以减轻抗菌剂耐药性的威胁，我们的研究结果表明，补充植物源性饲料添加剂可以导致商业肉鸡盲肠中的微生物组成相似，这表明使用替代产品可以导致功能修改，而不会增加抗菌剂耐药性的压力。
The prophylactic administration of ceftiofur to newly hatched chicks is a common practice in some hatcheries worldwide to mitigate early gastrointestinal infections caused by Enterobacteriaceae. In spite of the crucial role of the gut microbiome for the broiler's health, there is still limited information on how the microbial composition is affected by such procedure. We investigated the effects of posthatch prophylactic application of ceftiofur on the cecal microbiota of 14-day-old broilers fed regular or sanguinarine-supplemented diets. DNA samples were extracted from cecal contents, amplified for the V3-V4 regions of the microbial 16S rRNA gene, and sequenced in a high-throughput sequencing platform (Illumina MiSeq). After downstream bioinformatics and statistical analyses, our results demonstrated that both ceftiofur and sanguinarine treatments similarly increased the proportions of the phylum Bacteroidetes and the genera Bacteroides and Megamonas, whereas reduced the relative abundances of Firmicutes and Lachnospiraceae in the ceca of the birds. Such changes are probably associated with increased carbohydrate fermentation processes favoring the production of short-chain fatty acids. This was also corroborated by the functional prediction findings, which suggest an increase in some metabolic pathways associated with digestibility in broilers receiving ceftiofur. Considering that antimicrobial stewardship in animal production systems is strongly needed to mitigate the threat of antimicrobial resistance, our findings show that supplementation with a phytogenic feed additive can lead to a similar microbial composition in the ceca of commercial broiler chickens, suggesting that the use of alternative products could lead to functional modifications without increasing pressure for antimicrobial resistance.
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影响因子：
7.3
作者：
Lerner A;Matthias T;Aminov R
通讯作者：
Aminov R
影响因子：
3.7
作者：
Foster, Derek M.;Jacob, Megan E.;Papich, Mark G.
通讯作者：
Papich, Mark G.
影响因子：
56.9
作者：
Gill, Steven R.;Pop, Mihai;Nelson, Karen E.
通讯作者：
Nelson, Karen E.
影响因子：
3.7
作者：
Costa MC;Bessegatto JA;Alfieri AA;Weese JS;Filho JA;Oba A
通讯作者：
Oba A
影响因子：
2.7
作者：
Crisol-Martínez E;Stanley D;Geier MS;Hughes RJ;Moore RJ
通讯作者：
Moore RJ