A Microfluidic Method for the Selection of Undifferentiated Human Embryonic Stem Cells and in Situ Analysis.
A Microfluidic Method for the Selection of Undifferentiated Human Embryonic Stem Cells and in Situ Analysis.
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DOI：
10.1007/s10404-014-1485-9
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发表时间：
2015-05
影响因子：
2.8
通讯作者：
Sohn LL
中科院分区：
文献类型：
--
作者：
Jabart E;Rangarajan S;Lieu C;Hack J;Conboy I;Sohn LL
Conventional cell-sorting methods such as fluorescence-activated cell sorting (FACS) or magnetic-activated cell sorting (MACS) can suffer from certain shortcomings such as lengthy sample preparation time, cell modification through antibody labeling, and cell damage due to exposure to high shear forces or to attachment of superparamagnetic Microbeads. In light of these drawbacks, we have recently developed a label-free, microfluidic platform that can not only select cells with minimal sample preparation but also enable analysis of cells in situ. We demonstrate the utility of our platform by successfully isolating undifferentiated human embryonic stem cells (hESCs) from a heterogeneous population based on the undifferentiated stem-cell marker SSEA-4. Importantly, we show that, in contrast to MACS or FACS, cells isolated by our method have very high viability (~90%). Overall, our platform technology could likely be applied to other cell types beyond hESCs and to a variety of heterogeneous cell populations in order to select and analyze cells of interest.
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胚胎干细胞中细胞表面蛋白的整体表达
影响因子：
3.7
作者：
Gu B;Zhang J;Wang W;Mo L;Zhou Y;Chen L;Liu Y;Zhang M
通讯作者：
Zhang M
影响因子：
3.7
作者：
Emre N;Vidal JG;Elia J;O'Connor ED;Paramban RI;Hefferan MP;Navarro R;Goldberg DS;Varki NM;Marsala M;Carson CT
通讯作者：
Carson CT
DOI：
10.1007/978-1-60761-369-5_13
发表时间：
2010-01-01
期刊：
HUMAN EMBRYONIC STEM CELL PROTOCOLS, SECOND EDITION
影响因子：
--
作者：
Liu, Ying;Lakshmipathy, Uma;Chesnut, Jonathan D.
通讯作者：
Chesnut, Jonathan D.
影响因子：
9.2
作者：
Eiges, R;Schuldiner, M;Benvenisty, N
通讯作者：
Benvenisty, N
影响因子：
3.4
作者：
Armstrong, JK;Wenby, RB;Fisher, TC
通讯作者：
Fisher, TC