Dysregulation of Lipid Metabolism in Aging Meibomian Glands and Its Molecular Markers.
Dysregulation of Lipid Metabolism in Aging Meibomian Glands and Its Molecular Markers.
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DOI：
10.3390/ijms241713512
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发表时间：
2023-08-31
影响因子：
5.6
通讯作者：
Yuksel, Seher
中科院分区：
文献类型：
--
作者：
Butovich, Igor A.;Wilkerson, Amber;Yuksel, Seher
关键词：
外分泌睑板腺（MG）的主要功能是产生称为睑脂的富含脂质的分泌物，其在维持人类和大多数哺乳动物的眼表面稳态中起关键作用。睑脂的化学组成及其由MG产生的数量在很大程度上决定了它是否能够成功地发挥其作用。衰老通常与各种MG相关病理的发作相关。本研究的目的是确定衰老如何影响小鼠睑脂的化学成分和数量，并确定可能的衰老分子标记。使用液相色谱-高分辨率质谱法对小鼠MG脂质进行无偏、非靶向和靶向脂质组学评价，并使用主成分、正交投影至潜在结构判别式和偏最小二乘判别式分析对结果进行分析。我们发现，衰老导致MG中脂质代谢失调，以渐进的方式改变MG脂质（如蜡酯、三酰甘油和磷脂）的主要类别的比例。提出属于这些MG脂质组的几种脂质种类作为小鼠模型中衰老的明确标志物。
The main function of exocrine Meibomian glands (MGs) is to produce a lipid-rich secretion called meibum which plays a critical role in maintaining the ocular surface homeostasis of humans and most mammals. The chemical composition of meibum, and its quantity produced by MGs, largely determine whether it can fulfill its role successfully. Aging was frequently associated with the onset of various MG-related pathologies. The goal of this study was to determine how aging affects the chemical composition and quantity of meibum in mice, and identify possible molecular markers of aging. Unbiased, untargeted and targeted lipidomic evaluation of mouse MG lipids was conducted using liquid chromatography—high-resolution mass spectrometry, and the results were analyzed using Principal Component, Orthogonal Projections to Latent Structures Discriminant, and Partial Least Square Discriminant Analyses. We found that aging leads to dysregulation of lipid metabolism in MGs, changing the ratios of major classes of MG lipids (such as wax esters, triacylglycerols, and phospholipids) in a progressive manner. Several lipid species that belong to these groups of MG lipids are proposed as clear markers of aging in a mouse model.
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影响因子：
3.9
作者：
Ding, Juan;Sullivan, David A.
通讯作者：
Sullivan, David A.
影响因子：
5.6
作者：
通讯作者：
--
影响因子：
4.4
作者：
Borchman, Douglas;Foulks, Gary N.;Milliner, Sarah E.
通讯作者：
Milliner, Sarah E.
影响因子：
4.4
作者：
Butovich, Igor A.;Arciniega, Juan C.;Molai, Mike
通讯作者：
Molai, Mike
DOI：
10.1016/j.jtos.2018.10.003
发表时间：
2019-01
期刊：
The ocular surface
影响因子：
--
作者：
Paugh JR;Alfonso-Garcia A;Nguyen AL;Suhalim JL;Farid M;Garg S;Tao J;Brown DJ;Potma EO;Jester JV
通讯作者：
Jester JV