Trajectory mapping of the early Drosophila germline reveals controls of zygotic activation and sex differentiation.
Trajectory mapping of the early Drosophila germline reveals controls of zygotic activation and sex differentiation.
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DOI：
10.1101/gr.271148.120
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发表时间：
2021-06
期刊：
影响因子：
7
通讯作者：
Yang SY
中科院分区：
文献类型：
--
作者：
Li YR;Lai HW;Huang HH;Chen HC;Fugmann SD;Yang SY
黑腹果蝇的生殖细胞在受精后不久就被指定为母本，并且在转录上处于静止状态，直到它们的合子基因组被激活以维持进一步的发育。为了了解这一过程的分子基础，我们分析了早期男性和女性生殖细胞在单细胞水平上的进展转录组之间的种系规范和合并与体细胞性腺细胞。我们的数据全面覆盖合子激活在种系基因组中，并表现出种系限制性表达的基因的分析表明，聚合酶暂停和差异RNA稳定性是重要的机制，建立基因表达差异之间的种系和索马。此外，我们观察到一个立即之间的男性和女性生殖细胞的合子转录开始分叉。两种性别之间的主要差异是女性X染色体表达相对于男性升高，表明剂量补偿不完全，少数选择基因表现出更高的表达增加。这些表明雄性程序是生殖系中的默认模式，其被驱动为具有第二X染色体的雌性发育。
Germ cells in Drosophila melanogaster are specified maternally shortly after fertilization and are transcriptionally quiescent until their zygotic genome is activated to sustain further development. To understand the molecular basis of this process, we analyzed the progressing transcriptomes of early male and female germ cells at the single-cell level between germline specification and coalescence with somatic gonadal cells. Our data comprehensively cover zygotic activation in the germline genome, and analyses on genes that exhibit germline-restricted expression reveal that polymerase pausing and differential RNA stability are important mechanisms that establish gene expression differences between the germline and soma. In addition, we observe an immediate bifurcation between the male and female germ cells as zygotic transcription begins. The main difference between the two sexes is an elevation in X Chromosome expression in females relative to males, signifying incomplete dosage compensation, with a few select genes exhibiting even higher expression increases. These indicate that the male program is the default mode in the germline that is driven to female development with a second X Chromosome.
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DOI：
10.1093/bioinformatics/btr064
发表时间：
2011-04-01
期刊：
Bioinformatics (Oxford, England)
影响因子：
--
作者：
Grant CE;Bailey TL;Noble WS
通讯作者：
Noble WS
影响因子：
64.8
作者：
Cao, Junyue;Spielmann, Malte;Shendure, Jay
通讯作者：
Shendure, Jay
DOI：
10.1242/dev.113993
发表时间：
2015-08-15
期刊：
Development (Cambridge, England)
影响因子：
--
作者：
Johnson AD;Alberio R
通讯作者：
Alberio R
影响因子：
3.3
作者：
Meiklejohn CD;Presgraves DC
通讯作者：
Presgraves DC
影响因子：
64.5
作者：
LEHMANN, R;NUSSLEINVOLHARD, C
通讯作者：
NUSSLEINVOLHARD, C