The replicase gene of avian coronavirus infectious bronchitis virus is a determinant of pathogenicity.
The replicase gene of avian coronavirus infectious bronchitis virus is a determinant of pathogenicity.
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DOI：
10.1371/journal.pone.0007384
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发表时间：
2009-10-09
期刊：
影响因子：
3.7
通讯作者：
Britton P
中科院分区：
文献类型：
--
作者：
Armesto M;Cavanagh D;Britton P
我们先前已经证明，用来自强毒株（M41）的S基因替换来自传染性支气管炎病毒（IBV）的无毒毒株（Beaudette）的S基因，导致具有强毒株的体外细胞嗜性的重组病毒（BeauR-M41（S）），但其仍然是无毒的。为了研究是否有任何其他结构或辅助基因在致病性中起作用，我们现在用来自M41的基因替换了来自Beaudette菌株的基因。重组IBV实际上是一种嵌合病毒，其复制酶基因来自Beaudette，其余基因组来自M41。这表明，使用我们的瞬时显性选择方法，可以交换IBV基因组的大区域，约8.4kb。活的重组IBV的回收也证明，可以交换完整的复制酶基因，因为我们实际上用Beaudette衍生的基因替换了M41复制酶基因。嵌合病毒的分析表明，它是无毒的，这表明没有一个结构或辅助基因来源于强毒株的IBV能够恢复毒力，因此，与Beaudette株相关的毒力丧失存在于复制酶基因中。
We have previously demonstrated that the replacement of the S gene from an avirulent strain (Beaudette) of infectious bronchitis virus (IBV) with an S gene from a virulent strain (M41) resulted in a recombinant virus (BeauR-M41(S)) with the in vitro cell tropism of the virulent virus but that was still avirulent. In order to investigate whether any of the other structural or accessory genes played a role in pathogenicity we have now replaced these from the Beaudette strain with those from M41. The recombinant IBV was in effect a chimaeric virus with the replicase gene derived from Beaudette and the rest of the genome from M41. This demonstrated that it is possible to exchange a large region of the IBV genome, approximately 8.4 kb, using our transient dominant selection method. Recovery of a viable recombinant IBV also demonstrated that it is possible to interchange a complete replicase gene as we had in effect replaced the M41 replicase gene with the Beaudette derived gene. Analysis of the chimaeric virus showed that it was avirulent indicating that none of the structural or accessory genes derived from a virulent isolate of IBV were able to restore virulence and that therefore, the loss of virulence associated with the Beaudette strain resides in the replicase gene.
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