Detection of HPV DNA in esophageal cancer specimens from different regions and ethnic groups: a descriptive study.
Detection of HPV DNA in esophageal cancer specimens from different regions and ethnic groups: a descriptive study.
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不同地区和种族食管癌标本中 HPV DNA 的检测：一项描述性研究。
DOI：
10.1186/1471-2407-10-19
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发表时间：
2010-01-16
期刊：
影响因子：
3.8
通讯作者：
Ke Y
中科院分区：
文献类型：
--
作者：
Wang X;Tian X;Liu F;Zhao Y;Sun M;Chen D;Lu C;Wang Z;Shi X;Zhang Q;Zhang D;Shen Z;Li F;Harris CC;Cai H;Ke Y
在食道癌组织中已多次发现HPV。然而，报道的HPVDNA在这些肿瘤中的检测率有很大的差异。检测方法、样本类型和样本来源的地理区域的差异被认为是造成这种差异的潜在原因。对中国北部的安阳、中国南部的汕头、中国西部的新疆和美国4个不同民族的4个地区的435例食管癌标本进行了HPVL1DNA和HPV型别检测。用SPF1/GP6+引物检测HPVL1片段。采用基因特异性聚合酶链式反应进行HPV基因分型。在435份样本中，有244份(56.1%)HPVL1检测呈阳性。中国的三个地区之间以及中国与美国之间的发现率都没有明显差异。在L1阳性标本中检出HPV6、16、18、26、45、56、57和58。HPV16和57是所有地区最常见的类型，其次是HPV26和HPV18。HPV感染在食道癌中是常见的，不受地区和民族的影响。这项研究的发现提高了HPV参与食道癌发生的可能性。在更广泛的地理区域进行更大样本量的进一步调查可能是有必要的。
HPV has been found repeatedly in esophageal carcinoma tissues. However, reported detection rates of HPV DNA in these tumors have varied markedly. Differences in detection methods, sample types, and geographic regions of sample origin have been suggested as potential causes of this discrepancy. HPV L1 DNA and HPV genotypes were evaluated in 435 esophageal carcinoma specimens collected from four geographic regions with different ethnicities including Anyang in north China, Shantou in south China, Xinjiang in west China, and the United States. The HPV L1 fragment was detected using SPF1/GP6+ primers. HPV genotyping was performed using genotype specific PCR. Two hundred and forty four of 435 samples (56.1%) tested positive for HPV L1. Significant differences in detection rate were observed neither among the three areas of China nor between China and the US. HPV6, 16, 18, 26, 45, 56, 57, and 58 were identified in L1 positive samples. HPV16 and 57 were the most common types in all regions, followed by HPV26 and HPV18. HPV infection is common in esophageal carcinoma independent of region and ethnic group of origin. Findings in this study raise the possibility that HPV is involved in esophageal carcinogenesis. Further investigation with a larger sample size over broader geographic areas may be warranted.
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