The Saccharomyces cerevisiae histone chaperone Rtt106 mediates the cell cycle recruitment of SWI/SNF and RSC to the HIR-dependent histone genes.
The Saccharomyces cerevisiae histone chaperone Rtt106 mediates the cell cycle recruitment of SWI/SNF and RSC to the HIR-dependent histone genes.
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DOI：
10.1371/journal.pone.0021113
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发表时间：
2011
期刊：
影响因子：
3.7
通讯作者：
Prochasson P
中科院分区：
文献类型：
--
作者：
Ferreira ME;Flaherty K;Prochasson P
在酿酒酵母中，四个组蛋白基因对中的三个（HTA 1-HTB 1、HHT 1-HHF 1和HHT 2-HHF 2）受到HIR辅抑制子复合物的调节。组蛋白伴侣Rtt 106最近已被证明是存在于这些组蛋白基因位点在整个细胞周期中的HIR和Asf 1依赖的方式，并参与其转录抑制。SWI/SNF和RSC染色质重塑复合物都被招募到HR依赖性组蛋白基因中; SWI/SNF是它们在S期激活所必需的，而RSC涉及它们在S期外的抑制。尽管它们在组蛋白基因中的存在依赖于HIR复合物，但它们的特异性募集尚未得到很好的表征。在这项研究中，我们专注于表征组蛋白伴侣Rtt 106在SWI/SNF和RSC复合物向组蛋白基因的细胞周期依赖性募集中所发挥的作用。使用GST下拉和免疫共沉淀试验，我们表明，Rtt 106物理相互作用的SWI/SNF和RSC复合物在体外和体内。然后，我们研究了这种相互作用的功能，这些复合物的招聘依赖于HIR的组蛋白基因。使用染色质免疫沉淀试验（ChIP），我们发现，Rtt 106是重要的招聘SWI/SNF和RSC复合物的HIR依赖性组蛋白基因。此外，使用同步化细胞培养物，我们通过ChIP测定表明，Rtt 106依赖的SWI/SNF募集到这些组蛋白基因位点是细胞周期调节的，并且仅限于S期组蛋白基因表达峰值之前的G1期晚期。总之，这些数据强烈表明，组蛋白伴侣Rtt 106和SWI/SNF和RSC染色质重塑复合物之间的相互作用对于这两种复合物向HR依赖性组蛋白基因的细胞周期调节募集是重要的。
In Saccharomyces cerevisiae, three out of the four histone gene pairs (HTA1-HTB1, HHT1-HHF1, and HHT2-HHF2) are regulated by the HIR co-repressor complex. The histone chaperone Rtt106 has recently been shown to be present at these histone gene loci throughout the cell cycle in a HIR- and Asf1-dependent manner and involved in their transcriptional repression. The SWI/SNF and RSC chromatin remodeling complexes are both recruited to the HIR-dependent histone genes; SWI/SNF is required for their activation in S phase, whereas RSC is implicated in their repression outside of S phase. Even though their presence at the histone genes is dependent on the HIR complex, their specific recruitment has not been well characterized. In this study we focused on characterizing the role played by the histone chaperone Rtt106 in the cell cycle-dependent recruitment of SWI/SNF and RSC complexes to the histone genes. Using GST pull-down and co-immunoprecipitation assays, we showed that Rtt106 physically interacts with both the SWI/SNF and RSC complexes in vitro and in vivo. We then investigated the function of this interaction with respect to the recruitment of these complexes to HIR-dependent histone genes. Using chromatin immunoprecipitation assays (ChIP), we found that Rtt106 is important for the recruitment of both SWI/SNF and RSC complexes to the HIR-dependent histone genes. Furthermore, using synchronized cell cultures, we showed by ChIP assays that the Rtt106-dependent SWI/SNF recruitment to these histone gene loci is cell cycle regulated and restricted to late G1 phase just before the peak of histone gene expression in S phase. Overall, these data strongly suggest that the interaction between the histone chaperone Rtt106 and both the SWI/SNF and RSC chromatin remodeling complexes is important for the cell cycle regulated recruitment of these two complexes to the HIR-dependent histone genes.
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