Transformation of lipid bodies related to hydrocarbon accumulation in a green alga, Botryococcus braunii (Race B).
Transformation of lipid bodies related to hydrocarbon accumulation in a green alga, Botryococcus braunii (Race B).
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DOI：
10.1371/journal.pone.0081626
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Noguchi T
中科院分区：
文献类型：
--
作者：
Suzuki R;Ito N;Uno Y;Nishii I;Kagiwada S;Okada S;Noguchi T
菌落微藻布朗葡萄球菌主要在细胞外空间积累大量的烃;大多数其他产油微藻在细胞质中储存脂质。根据烃类的类型，布朗葡萄球菌分为三个主要的小种（A、B和L）。作为石油的替代品，品种B吸引了最多的关注，因为它的烃含量比其他品种高，并且它的烃组分葡萄球菌烯和甲基角鲨烯都可以容易地转化为生物燃料。我们研究了种族B，用荧光和电子显微镜，并阐明了细胞周期中发生的胞外碳氢化合物积累的阶段，在相关的行为和结构变化的脂质体和讨论的发展的藻类群体。细胞分裂后，在子细胞的基底外侧区域，细胞表面发生新的脂质积累。虽然在整个细胞周期中观察到脂质体，但它们的大小和内含物是动态变化的。当细胞开始分裂时，脂质体的大小和内含物增加，直到脂质的细胞外积累开始。大部分脂质随着细胞外积累而从细胞质中消失，然后重新形成。因此，我们推测，在B的生长过程中产生的脂质体。Braunii与脂质分泌有关。细胞表面分泌的新脂质形成了一层层的油滴，最深可达六层，并融合形成扁平的连续薄片。在连续的细胞分裂过程中，结合一对子细胞的片层保持不变，并且随着细胞数量的增加，殖民地的大小增加。
The colonial microalga Botryococcus braunii accumulates large quantities of hydrocarbons mainly in the extracellular space; most other oleaginous microalgae store lipids in the cytoplasm. Botryococcus braunii is classified into three principal races (A, B, and L) based on the types of hydrocarbons. Race B has attracted the most attention as an alternative to petroleum by its higher hydrocarbon contents than the other races and its hydrocarbon components, botryococcenes and methylsqualenes, both can be readily converted into biofuels. We studied race B using fluorescence and electron microscopy, and clarify the stage when extracellular hydrocarbon accumulation occurs during the cell cycle, in a correlation with the behavior and structural changes of the lipid bodies and discussed development of the algal colony. New accumulation of lipids on the cell surface occurred after cell division in the basolateral region of daughter cells. While lipid bodies were observed throughout the cell cycle, their size and inclusions were dynamically changing. When cells began dividing, the lipid bodies increased in size and inclusions until the extracellular accumulation of lipids started. Most of the lipids disappeared from the cytoplasm concomitant with the extracellular accumulation, and then reformed. We therefore hypothesize that lipid bodies produced during the growth of B. braunii are related to lipid secretion. New lipids secreted at the cell surface formed layers of oil droplets, to a maximum depth of six layers, and fused to form flattened, continuous sheets. The sheets that combined a pair of daughter cells remained during successive cellular divisions and the colony increased in size with increasing number of cells.
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影响因子：
3.7
作者：
Furuhashi K;Saga K;Okada S;Imou K
通讯作者：
Imou K
影响因子：
3.3
作者：
Eroglu, Ela;Okada, Shigeru;Melis, Anastasios
通讯作者：
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影响因子：
4.3
作者：
BERGFELD, R;HONG, YN;SCHOPFER, P
通讯作者：
SCHOPFER, P
影响因子：
2.9
作者：
BERKALOFF, C;ROUSSEAU, B;CHIRAC, C
通讯作者：
CHIRAC, C
影响因子：
--
作者：
Hirose, Mana;Mukaida, Fukiko;Noguchi, Tetsuko
通讯作者：
Noguchi, Tetsuko