Seawater-cultured Botryococcus braunii for efficient hydrocarbon extraction.
Seawater-cultured Botryococcus braunii for efficient hydrocarbon extraction.
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DOI：
10.1371/journal.pone.0066483
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发表时间：
2013
期刊：
影响因子：
3.7
通讯作者：
Imou K
中科院分区：
文献类型：
--
作者：
Furuhashi K;Saga K;Okada S;Imou K
作为生物燃料的潜在来源，绿色菌落微藻布朗葡萄球菌（Botryococcus braunii）产生大量积聚在细胞外基质中的烃。通常，需要预处理如干燥或加热湿藻类以从B充分回收烃。Braunii使用有机溶剂。在这项研究中，昭和株的B。通过供应人工海水、天然海水或NaCl，在源自改良Chu 13培养基的培养基中培养布氏藻。在渗透压相当于1/4海水的培养基中培养一段时间后，通过简单地将完整的湿藻与正己烷混合而不进行任何预处理，获得了超过90%的烃回收率，并且使用本培养条件的结果表明了烃挤奶的潜力。海水用于从布朗葡萄藻（Botryococcus braunii）中高效提取烃。在用海水或NaCl制备的培养基中培养该真菌。在不进行任何预处理的情况下，烃回收率超过90%。
As a potential source of biofuel, the green colonial microalga Botryococcus braunii produces large amounts of hydrocarbons that are accumulated in the extracellular matrix. Generally, pretreatment such as drying or heating of wet algae is needed for sufficient recoveries of hydrocarbons from B. braunii using organic solvents. In this study, the Showa strain of B. braunii was cultured in media derived from the modified Chu13 medium by supplying artificial seawater, natural seawater, or NaCl. After a certain period of culture in the media with an osmotic pressure corresponding to 1/4-seawater, hydrocarbon recovery rates exceeding 90% were obtained by simply mixing intact wet algae with n-hexane without any pretreatments and the results using the present culture conditions indicate the potential for hydrocarbon milking. Seawater was used for efficient hydrocarbon extraction from Botryococcus braunii. The alga was cultured in media prepared with seawater or NaCl. Hydrocarbon recovery rate exceeding 90% was obtained without any pretreatment.
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