Expression of glycolipids bearing Lewis phenotypes in tissues and cultured cells of human gynecological cancers.
Expression of glycolipids bearing Lewis phenotypes in tissues and cultured cells of human gynecological cancers.
复制标题
DOI：
10.1111/j.1349-7006.2002.tb01215.x
复制
发表时间：
2002-10
期刊：
影响因子：
--
通讯作者：
Iwamori M
中科院分区：
文献类型：
--
作者：
Takehara K;Kubushiro K;Kiguchi K;Ishiwata I;Tsukazaki K;Nozawa S;Iwamori M
Leb（Lewis 抗原-b）或 LeY 在人类结直肠癌中的转化相关表达已得到充分描述。为了检查人类妇科癌衍生细胞中具有 Lewis 表型的鞘糖脂 (GSL) 的表达，我们测定了所有 GSL 的浓度。尽管在低分化型子宫内膜腺癌建立的 HEC-108 细胞中既不存在 Leb 也不存在 LeY，但其他中分化或高分化类型的细胞系表达 Leb 或 LeY，或两者都表达，浓度为每毫克干细胞 0.01 至 0.03 μg，占总 GSL 的 0.3 至 1.3%。在宫颈癌和卵巢癌来源的细胞系中，Lewis表型往往由nLc4Cer携带，它在没有唾液酸化或岩藻糖基化的细胞中积累。这些结果表明 Leb 或 LeY 表型的表达强烈依赖于提供前体 GSL 的代谢能力。单克隆抗体MSN-1成功检测到了Leb和LeY，这是同时检测Leb和LeY的有用探针。应用MSN-1时，在高分化型和中分化型子宫内膜腺癌患者的组织中检测到Leb或LeY，浓度为每毫克干组织0.01至0.04 μg，但在低分化型组织中未检测到。正常卵泡期和黄体期子宫内膜也含有抗原，但抗原表达浓度和频率低于高分化和中分化子宫内膜腺癌。
Transformation‐associated expression of Leb (Lewis antigen‐b) or LeY in human colorectal carcinomas has been well described. To examine the expression of glycosphingolipids (GSLs) bearing Lewis‐phenotypes in human gynecological carcinoma‐derived cells, we determined the concentrations of all GSLs. Although neither Leb nor LeY was present in HEC‐108 cells established from the poorly differentiated type of endometrial adenocarcinoma, other cell lines from moderately or well‐differentiated types expressed either Leb or LeY, or both, at concentrations of 0.01 to 0.03 μg per mg of dry cells, which comprised 0.3 to 1.3% of the total GSLs. In the cervical and ovarian carcinoma‐derived cell lines, Lewis phenotypes tended to be carried by nLc4Cer, which was accumulated in the cells without sialylation or fucosylation. These results indicated that expression of Leb‐ or LeY‐phenotypes was strongly dependent on the metabolic ability to supply the precursor GSLs. Both Leb and LeY were successfully detected by monoclonal antibody MSN‐1, which was a useful probe for the simultaneous detection of Leb and LeY. On application of MSN‐1, either Leb or LeY was detected in tissues from patients with well‐ and moderately differentiated types of endometrial adenocarcinoma at concentrations of 0.01 to 0.04 μg per mg of dry tissues, but not in the tissues of poorly differentiated type. Normal endometria at the follicular and luteal phases also contained the antigens, but the concentrations and the frequency of antigen expression were lower than those in the well‐ and moderately differentiated types of endometrial adenocarcinoma.
登录
查看更多内容
影响因子：
4.7
作者：
ISHIWATA, I;ISHIWATA, C;ISHIKAWA, H
通讯作者：
ISHIKAWA, H
影响因子：
56.9
作者：
KOPROWSKI, H;HERLYN, M;SEARS, HF
通讯作者：
SEARS, HF
影响因子：
2.7
作者：
IWAMORI, M;SAKAYORI, M;NAGAI, Y
通讯作者：
NAGAI, Y
影响因子：
2
作者：
Baldus, SE;Hanisch, FG;Dienes, HP
通讯作者：
Dienes, HP
DOI：
10.1046/j.1432-1327.2001.02254.x
发表时间：
2001-06-01
期刊：
EUROPEAN JOURNAL OF BIOCHEMISTRY
影响因子：
--
作者：
Liu, F;Qi, HL;Chen, HL
通讯作者：
Chen, HL