A Novel Aptamer-Imprinted Polymer-Based Electrochemical Biosensor for the Detection of Lead in Aquatic Products.
A Novel Aptamer-Imprinted Polymer-Based Electrochemical Biosensor for the Detection of Lead in Aquatic Products.
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DOI：
10.3390/molecules28010196
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发表时间：
2022-12-26
期刊：
影响因子：
4.6
通讯作者：
Xu, Fei
中科院分区：
文献类型：
--
作者：
Zhu, Nianxin;Liu, Xinna;Peng, Kaimin;Cao, Hui;Yuan, Min;Ye, Tai;Wu, Xiuxiu;Yin, Fengqin;Yu, Jinsong;Hao, Liling;Xu, Fei
水产品中铅污染是主要危害因素之一。适配型传感器具有良好的选择性，是一种很有前途的铅离子(Pb2+)检测方法，但易受pH影响。离子印迹聚合物(IIP)与核酸适体的结合可以提高其在不同pH条件下的稳定性。以适体印迹聚合物为识别元件，研制了一种新型的铅(II)电化学生物传感器。用金纳米粒子和适配子修饰玻碳电极。在铅诱导适配子形成G-四链构象后，电沉积壳聚糖-氧化石墨烯，并与戊二醛交联形成印迹层，提高了生物传感器的稳定性。在最佳实验条件下，电流信号变化量(∆I)与铅含量在0.0796.1~2.0μg/m L范围内呈良好的线性关系，检出限为0.0796μg/m L(S/N=3)。在其他干扰金属离子存在的情况下，该生物传感器对铅(II)的测定也表现出很高的选择性。同时，印迹层的稳定性使该传感器适用于pH为6.4-8.0的检测环境。此外，该传感器还成功地应用于螳螂虾中铅的检测。
Lead contamination in aquatic products is one of the main hazard factors. The aptasensor is a promising detection method for lead ion (Pb(II)) because of its selectivity, but it is easily affected by pH. The combination of ion-imprinted polymers(IIP) with aptamers may improve their stability in different pH conditions. This paper developed a novel electrochemical biosensor for Pb(II) detection by using aptamer-imprinted polymer as a recognition element. The glassy carbon electrode was modified with gold nanoparticles and aptamers. After the aptamer was induced by Pb(II) to form a G-quadruplex conformation, a chitosan-graphene oxide was electrodeposited and cross-linked with glutaraldehyde to form an imprint layer, improving the stability of the biosensor. Under the optimal experimental conditions, the current signal change (∆I) showed a linear correlation of the content of Pb(II) in the range of 0.1–2.0 μg/mL with a detection limit of 0.0796 μg/mL (S/N = 3). The biosensor also exhibited high selectivity for the determination of Pb(II) in the presence of other interfering metal ion. At the same time, the stability of the imprinted layer made the sensor applicable to the detection environment with a pH of 6.4–8.0. Moreover, the sensor was successfully applied to the detection of Pb(II) in mantis shrimp.
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