Functional neural differentiation of human adipose tissue-derived stem cells using bFGF and forskolin.
Functional neural differentiation of human adipose tissue-derived stem cells using bFGF and forskolin.
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DOI：
10.1186/1471-2121-11-25
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发表时间：
2010-04-16
期刊：
影响因子：
--
通讯作者：
Jeong HS
中科院分区：
文献类型：
--
作者：
Jang S;Cho HH;Cho YB;Park JS;Jeong HS
来源于脂肪组织的成体间充质干细胞（MSCs）在体外具有向间充质细胞、内胚层细胞和外胚层细胞分化的能力。我们将人脂肪组织来源的干细胞（hADSCs）作为MSC，并研究了这些细胞的神经分化潜能。从耳垂脂肪中提取的人脂肪干细胞在特定的培养条件下保持自我更新能力，并分化为脂肪细胞、成骨细胞或软骨细胞。在用bFGF和毛喉素进行神经诱导后，hADSCs在体外分化为包括神经元和胶质细胞在内的各种类型的神经细胞。在神经分化的hADSC（NI-hADSC）中，神经干细胞标志物（nestin）、神经元标志物（Tuj 1、MAP 2、NFL、NFM、NFH、NSE和NeuN）、星形胶质细胞标志物（GFAP）和少突胶质细胞标志物（CN）的免疫反应性比原代hADSC中显著增加。RT-PCR分析表明，NI-hADSCs中ABCG 2、nestin、Tuj 1、MAP 2、NFL、NFM、NSE、GAP 43、SNAP 25、GFAP和CNF 1的mRNA水平也显著升高。此外，NI-hADSCs获得神经元样功能，其特征在于在全细胞膜片钳记录下显示电压依赖性河豚毒素（TTX）敏感性钠电流、外向钾电流和显著的负静息膜电位。通过RT-PCR进一步检测显示NI-hADSC表达高水平的钠离子通道基因（SCN 5A）、钾离子通道基因（MaxiK、Kv4.2和EAG 2）和钙离子通道基因（CACNA 1C和CACNA 1G），这些基因在原代hADSC中组成型表达。此外，我们证明了Kv4.3和Kv 1，钾通道基因，和NE-Na，TTX敏感的钠通道基因，高度诱导神经分化后。这些综合结果表明，hADSCs具有与干细胞相同的自我更新能力和多能性，并且可以使用bFGF和毛喉素分化为功能性神经元。
Adult mesenchymal stem cells (MSCs) derived from adipose tissue have the capacity to differentiate into mesenchymal as well as endodermal and ectodermal cell lineage in vitro. We characterized the multipotent ability of human adipose tissue-derived stem cells (hADSCs) as MSCs and investigated the neural differentiation potential of these cells. Human ADSCs from earlobe fat maintained self-renewing capacity and differentiated into adipocytes, osteoblasts, or chondrocytes under specific culture conditions. Following neural induction with bFGF and forskolin, hADSCs were differentiated into various types of neural cells including neurons and glia in vitro. In neural differentiated-hADSCs (NI-hADSCs), the immunoreactivities for neural stem cell marker (nestin), neuronal markers (Tuj1, MAP2, NFL, NFM, NFH, NSE, and NeuN), astrocyte marker (GFAP), and oligodendrocyte marker (CNPase) were significantly increased than in the primary hADSCs. RT-PCR analysis demonstrated that the mRNA levels encoding for ABCG2, nestin, Tuj1, MAP2, NFL, NFM, NSE, GAP43, SNAP25, GFAP, and CNPase were also highly increased in NI-hADSCs. Moreover, NI-hADSCs acquired neuron-like functions characterized by the display of voltage-dependent tetrodotoxin (TTX)-sensitive sodium currents, outward potassium currents, and prominent negative resting membrane potentials under whole-cell patch clamp recordings. Further examination by RT-PCR showed that NI-hADSCs expressed high level of ionic channel genes for sodium (SCN5A), potassium (MaxiK, Kv4.2, and EAG2), and calcium channels (CACNA1C and CACNA1G), which were expressed constitutively in the primary hADSCs. In addition, we demonstrated that Kv4.3 and Eag1, potassium channel genes, and NE-Na, a TTX-sensitive sodium channel gene, were highly induced following neural differentiation. These combined results indicate that hADSCs have the same self-renewing capacity and multipotency as stem cells, and can be differentiated into functional neurons using bFGF and forskolin.
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影响因子：
4.9
作者：
Janjanin S;Djouad F;Shanti RM;Baksh D;Gollapudi K;Prgomet D;Rackwitz L;Joshi AS;Tuan RS
通讯作者：
Tuan RS
影响因子：
11.2
作者：
Bang, OY;Lee, JS;Lee, G
通讯作者：
Lee, G
影响因子：
5.2
作者：
Cho, KJ;Trzaska, KA;Rameshwar, P
通讯作者：
Rameshwar, P
影响因子：
4.8
作者：
Egusa, H;Schweizer, FE;Nishimura, I
通讯作者：
Nishimura, I
DOI：
10.1016/j.bbrc.2005.05.096
发表时间：
2005-07-22
影响因子：
3.1
作者：
Fujimura, J;Ogawa, R;Suzuki, H
通讯作者：
Suzuki, H