Alterations in the gut microbiome and metabolic profile in rats acclimated to high environmental temperature.
Alterations in the gut microbiome and metabolic profile in rats acclimated to high environmental temperature.
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适应高环境温度的大鼠肠道微生物组和代谢特征的变化
DOI：
10.1111/1751-7915.13772
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发表时间：
2022-01
影响因子：
5.7
通讯作者：
Niu C
中科院分区：
文献类型：
--
作者：
Cao Y;Liu Y;Dong Q;Wang T;Niu C
热驯化（HA）是通过诱导积极的生理适应来提高热胁迫耐受性的最佳策略。有证据表明，肠道微生物群在HA的发展中起着重要作用，肠道微生物群的调节可以提高对热暴露的耐受性，降低热疾病的风险。在这项研究中，我们首次应用16S rRNA基因测序和非靶向液相色谱-质谱（LC - MS）代谢组学来探索HA后大鼠肠道微生物组和粪便代谢谱的变化。HA受试者的肠道菌群表现出更高的多样性和更丰富的微生物。透明质酸改变了肠道菌群组成，乳酸杆菌属（一种主要的益生菌）和示波螺旋菌属显著增加，而蓝杆菌属和异源杆菌属显著减少。HA后粪便代谢组也发生了显著变化，在13种受干扰的代谢物中，(S)‐AL 8810和celastrol升高。此外，2个增加属与2个上调代谢物呈正相关，与其他11个下调代谢物呈负相关，而2个减少属与上调/下调代谢物的相关性完全相反。综上所述，HA处理28天后，肠道微生物群落结构和粪便代谢组均得到改善。这些发现为改善肠道微生物群及其作为透明质酸提供热应激保护的潜在机制的功能提供了新的见解。有证据表明，肠道微生物组在HA的发展中起着重要作用。在这项研究中，我们应用16S rRNA基因测序和非靶向液相色谱-质谱（LC - MS）代谢组学来探索HA后大鼠肠道微生物组和粪便代谢谱的变化。我们的研究首次证明了透明质酸对肠道微生物组和粪便代谢组有显著影响，这可能是透明质酸保护机体免受热应激的潜在机制。
Heat acclimation (HA) is the best strategy to improve heat stress tolerance by inducing positive physiological adaptations. Evidence indicates that the gut microbiome plays a fundamental role in the development of HA, and modulation of gut microbiota can improve tolerance to heat exposure and decrease the risks of heat illness. In this study, for the first time, we applied 16S rRNA gene sequencing and untargeted liquid chromatography–mass spectrometry (LC‐MS) metabolomics to explore variations in the gut microbiome and faecal metabolic profiles in rats after HA. The gut microbiota of HA subjects exhibited higher diversity and richer microbes. HA altered the gut microbiota composition with significant increases in the genera Lactobacillus (a major probiotic) and Oscillospira alongside significant decreases in the genera Blautia and Allobaculum. The faecal metabolome was also significantly changed after HA, and among the 13 perturbed metabolites, (S)‐AL 8810 and celastrol were increased. Moreover, the two increased genera were positively correlated with the two upregulated metabolites and negatively correlated with the other 11 downregulated metabolites, while the correlations between the two decreased genera and the upregulated/downregulated metabolites were completely contrary. In summary, both the structure of the gut microbiome community and the faecal metabolome were improved after 28 days of HA. These findings provide novel insights regarding the improvement of the gut microbiome and its functions as a potential mechanism by which HA confers protection against heat stress. Evidence indicates that the gut microbiome plays a fundamental role in the development of HA. In this study, we applied 16S rRNA gene sequencing and untargeted liquid chromatography‐mass spectrometry (LC‐MS) metabolomics to explore variations in the gut microbiome and fecal metabolic profiles in rats after HA. Our study demonstrates for the first time that HA has a significant effect on the gut microbiome and fecal metabolome, and this may be a potential mechanism by which HA confers protection against heat stress.
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影响因子：
15.5
作者：
Hu J;Raikhel V;Gopalakrishnan K;Fernandez-Hernandez H;Lambertini L;Manservisi F;Falcioni L;Bua L;Belpoggi F;L Teitelbaum S;Chen J
通讯作者：
Chen J
影响因子：
5.8
作者：
Gu, Zuguang;Eils, Roland;Schlesner, Matthias
通讯作者：
Schlesner, Matthias
影响因子：
4.6
作者：
Chen S;Wang J;Peng D;Li G;Chen J;Gu X
通讯作者：
Gu X
影响因子：
4.1
作者：
Chong, Jasmine;Yamamoto, Mai;Xia, Jianguo
通讯作者：
Xia, Jianguo
影响因子：
5.1
作者：
Gophna, Uri;Konikoff, Tom;Nielsen, Henrik Bjorn
通讯作者：
Nielsen, Henrik Bjorn