Cytoprotective Antioxidant, Anti-Inflammatory, and Antifibrotic Impact of Celery Seed Oil and Manuka Honey Against Cyclophosphamide-Induced Cystitis in Rabbits.
Cytoprotective Antioxidant, Anti-Inflammatory, and Antifibrotic Impact of Celery Seed Oil and Manuka Honey Against Cyclophosphamide-Induced Cystitis in Rabbits.
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DOI：
10.1155/2022/2863023
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发表时间：
2022
期刊：
影响因子：
--
通讯作者：
Elsayed AM
中科院分区：
文献类型：
--
作者：
Mousa AM;Allemailem KS;Alhumaydhi FA;Alrumaihi F;Almatroudi A;Aljasir M;Alwashmi ASS;Al Rugaie O;Soliman KEA;Aljohani ASM;Al Abdulmonem W;Ahmed AA;Khan A;Khan MA;AlSuhaymi N;Alsugoor MH;Al-Megrin WA;Elsayed AM
使用环磷酰胺（CP）治疗的患者通常患有严重的出血性膀胱炎（HC）。我们之前的研究表明，美司钠+芹菜联合疗法可部分改善 HC。因此，迫切需要寻找替代方案来获得针对 CP 诱导的 HC 的完全保护。目前的研究调查了美司钠+芹菜籽油 (MCSO) 或美司钠+麦卢卡蜂蜜 (MMH) 联合疗法对成年雄性兔子 CP 诱导的 HC 的影响。将四十只兔子分成相等的四组并治疗三周。对照组（G1）接受蒸馏水，第二组（G2）接受CP（50mg/kg/周）。第三组（G3）接受CP + MCSO（CPMCSO方案），第四组（G4）接受CP + MMH（CPMMH方案）。对膀胱 (UB) 标本进行处理，通过组织病理学、免疫组织化学、超微结构和生化研究来评估 UB 的变化。在 G2 中，CP 引发 HC 特征（尿路上皮坏死、溃疡和腐肉）、UB 纤维化和 TNF-α 免疫表达。此外，CP还能降低G2兔抗氧化酶（GPx1、SOD3和CAT）的活性，并升高血清中NF-κB、TNF-α、IL-1B和IL-6细胞因子的水平。相比之下，与 CPMCSO 方案在 G3 中的部分保护相比，CPMCSO 方案在 G4 兔中引起 UB 对 HC 的保护显着增加。因此，我们的研究首次表明，新型 CPMMH 方案通过结合抗氧化、抗炎和抗纤维化特性，对 CP 诱导的 HC 具有完全的 UB 保护作用。
Patients treated with cyclophosphamide (CP) usually suffer from severe hemorrhagic cystitis (HC). Our previous study exhibited that mesna + celery cotherapy partially ameliorated HC. Therefore, there is a substantial need to seek alternative regimens to get complete protection against CP-induced HC. The current study investigated the effects of mesna + celery seed oil (MCSO) or mesna + manuka honey (MMH) cotherapy against CP-induced HC in adult male rabbits. The forty rabbits were divided into four equal groups and treated for three weeks. The control group (G1) received distilled water and the second group (G2) received CP (50 mg/kg/week). The third group (G3) received CP + MCSO (CPMCSO regimen), and the fourth group (G4) received CP + MMH (CPMMH regimen). The urinary bladder (UB) specimens were processed to evaluate UB changes through histopathological, immunohistochemical, ultrastructural, and biochemical investigations. In G2, CP provoked HC features (urothelial necrosis, ulceration, and sloughing), UB fibrosis, and TNF-α immunoexpression. Besides, CP reduced the activity of antioxidant enzymes (GPx1, SOD3, and CAT) and elevated the serum levels of NF-κB, TNF-α, IL-1B, and IL-6 cytokines in G2 rabbits. In contrast, the CPMMH regimen caused significant increments of UB protection against HC in G4 rabbits compared to the partial protection by the CPMCSO regimen in G3. Therefore, our study indicated for the first time that the novel CPMMH regimen resulted in complete UB protection against CP-induced HC via combined antioxidant, anti-inflammatory, and antifibrotic properties.
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影响因子：
--
作者：
Al-Waili N;Salom K;Al-Ghamdi AA
通讯作者：
Al-Ghamdi AA
DOI：
10.3390/foods3030420
发表时间：
2014-07-21
期刊：
Foods (Basel, Switzerland)
影响因子：
--
作者：
Alvarez-Suarez JM;Gasparrini M;Forbes-Hernández TY;Mazzoni L;Giampieri F
通讯作者：
Giampieri F
影响因子：
1.9
作者：
El-Shinnawy, Nashwa A.
通讯作者：
El-Shinnawy, Nashwa A.
影响因子：
--
作者：
Kooti, Wesam;Moradi, Maryam;Ghafourian, Mehri
通讯作者：
Ghafourian, Mehri
影响因子：
2.1
作者：
Lee, Joo Yong;Chang, Jin Seok;Cho, Kang Su
通讯作者：
Cho, Kang Su