The effects of D-penicillamine on a murine model of oxygen-induced retinopathy.
The effects of D-penicillamine on a murine model of oxygen-induced retinopathy.
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DOI：
10.1016/j.jaapos.2011.04.005
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发表时间：
2011-08
期刊：
影响因子：
1.6
通讯作者：
Moreau, Annie
中科院分区：
文献类型：
--
作者：
Siatkowski, R. Michael;Yanovitch, Tammy L.;Ash, John D.;Moreau, Annie
目的：探讨D-青霉胺(DPA)对氧性视网膜病变小鼠模型视网膜新生血管的影响。出生后第7天，16只小鼠腹腔注射DPA 300 mg/kg/d，连续3天，然后再注射50 mg/kg/d，连续7天。另一组7只小鼠，先给予DPA 600 mg/kg/d，连续3天，然后再给予100 mg/kg/d，连续7天。第三组14只小鼠每天注射1,500 mg/kg DPA，连续2天；对照组17只小鼠注射磷酸盐缓冲盐水(PBS)。另取15只小鼠右眼玻璃体腔注射1μL 100 mg/mLDPA，左眼玻璃体腔注射1μL PBS作为对照。将各组动物置于75%氧气室中7天，然后在室内空气中放置3天，然后处死动物，摘除眼球。视网膜染色和平铺，通过量化新生血管芽来确定视网膜新生血管的严重程度。经腹膜腔注射后，无论DPA剂量如何，DPA组和PBS组肾小球和肾小管的平均数目相似(P=1.0)。1,500毫克/公斤/天的剂量被证明是一致致命的。玻璃体内注射后，DPA组和PBS组的平均肾小球数目(P=0.16)和平均肾小管数目(P=0.7)相似。无论是在腹膜内还是玻璃体内注射DPA都不能抑制氧诱导的视网膜病变小鼠模型中的视网膜新生血管。
To determine the effect of intraperitoneal and intravitreal D-penicillamine (DPA) on retinal neovascularization in a murine model of oxygen-induced retinopathy. On postnatal day 7, 16 mice were injected intraperitoneally with 300 mg/kg/day DPA for 3 days followed by 50 mg/kg/day for 7 days. A different group of 7 mice were injected intraperitoneally with 600 mg/kg/day DPA for 3 days followed by 100 mg/kg/day for 7 days. A third group of 14 mice were injected with 1,500 mg/kg/day DPA for 2 days; a control cohort of 17 mice received intraperitoneal phosphate-buffered saline (PBS). An additional 15 mice underwent intravitreal injection of 1 μL of 100 mg/mL DPA in the right eye and 1 μL PBS intravitreally in the left eye as a control. All groups were placed in a 75% oxygen chamber for 7 days then room air for 3 days before being sacrificed and enucleated. The retinas were stained and flat-mounted to determine the severity of retinal neovascularization by quantifying neovascular buds. After intraperitoneal injection, the mean number of glomeruli and tubules was similar in the DPA and PBS groups (P = 1.0), regardless of DPA dosage. The dosage of 1,500 mg/kg/day proved to be uniformly lethal. After intravitreal injections, the mean number of glomeruli (P = 0.16) and tubules (P = 0.7) were similar in the DPA and PBS groups. Neither intraperitoneal nor intravitreal injection of DPA inhibits retinal neovascularization in a murine model of oxygen-induced retinopathy.
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影响因子：
2.9
作者：
Christensen, R. D.;Alder, S. C.;Baer, V. L.
通讯作者：
Baer, V. L.
影响因子：
158.5
作者：
Askie, LM;Henderson-Smart, DJ;Simpson, JM
通讯作者：
Simpson, JM
影响因子：
1.4
作者：
PENN, JS
通讯作者：
PENN, JS
影响因子：
--
作者：
PHELPS, DL;ROSENBAUM, AL
通讯作者：
ROSENBAUM, AL
DOI：
10.1159/000087586
发表时间：
2005-01-01
期刊：
BIOLOGY OF THE NEONATE
影响因子：
--
作者：
Saugstad, OD
通讯作者：
Saugstad, OD