Relationships between spinal cord blood flow measured with flow-sensitive alternating inversion recovery (FAIR) and neurobehavioral outcomes in rat spinal cord injury.
Relationships between spinal cord blood flow measured with flow-sensitive alternating inversion recovery (FAIR) and neurobehavioral outcomes in rat spinal cord injury.
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DOI：
10.1016/j.mri.2021.02.004
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发表时间：
2021-05
影响因子：
2.5
通讯作者：
Budde MD
中科院分区：
文献类型：
--
作者：
Lee S;Wilkins N;Schmit BD;Kurpad SN;Budde MD
在创伤损伤的脊髓中，血流灌注减少被认为是导致继发性组织损伤的原因之一，而恢复血流灌注被认为是一个有前途的治疗靶点。然而，非侵入性监测脊髓血流灌注的方法有限。为大脑建立的核磁共振成像(MRI)技术还没有被常规地应用于脊髓。本研究的目的是在大鼠胸段脊髓挫伤(SCI)模型中，采用两种不同标记位置的流动交替敏感反转恢复(FIRE)技术，研究脊髓血流量(SCBF)与损伤严重程度的关系。将SCBF作为严重程度的标志与T1图和经滤过的平行表观弥散系数的脊髓优化弥散加权成像(DWI)进行比较。38只大鼠造成不同程度的T10挫伤(8只假挫伤，10只轻度挫伤，10只中度挫伤，10只重度挫伤)，伤后1d行MRI检查，伤后28d用Basso，Beattie，Bresna han(BBB)运动功能评分进行神经学随访。使用损伤中心的全脊髓感兴趣区，SCBF随着损伤严重程度的增加而降低，并与伤后28天的BBB评分显著相关。重要的是，损伤后脊髓损伤后动脉通过时间(ATT)的估计没有改变，这表明在脊髓急性创伤性损伤的情况下，优化的公平方案来测量SCBF提供了更多的价值。T1-松弛时间常数与损伤严重程度密切相关，其变化幅度大于SCBF或DWI测量。这些发现表明，损伤后脊髓的血流灌注减少可以非侵入性地监测，多参数MRI对血流灌注、扩散和松弛的评估捕捉到了临床前损伤病理生理学的独特特征。
In the traumatically injured spinal cord, decreased perfusion is believed to contribute to secondary tissue damage beyond the primary mechanical impact, and restoration of perfusion is believed to be a promising therapeutic target. However, methods to monitor spinal cord perfusion non-invasively are limited. Perfusion magnetic resonance imaging (MRI) techniques established for the brain have not been routinely adopted to the spinal cord. The purpose of this study was to examine the relationship between spinal cord blood flow (SCBF) and injury severity in a rat thoracic spinal cord contusion injury (SCI) model using flow-alternating sensitive inversion recovery (FAIR) with two variants of the label position. SCBF as a marker of severity was compared to T1 mapping and to spinal cordoptimized diffusion weighted imaging (DWI) with filtered parallel apparent diffusion coefficient. Thirty-eight rats underwent a T10 contusion injury with varying severities (8 sham; 10 mild; 10 moderate; 10 severe) with MRI performed at 1 day post injury at the lesion site and follow-up neurological assessments using the Basso, Beattie, Bresnahan (BBB) locomotor scoring up to 28 days post injury. Using whole-cord regions of interest at the lesion epicenter, SCBF was decreased with injury severity and had a significant correlation with BBB scores at 28 days post injury. Importantly, estimates of arterial transit times (ATT) in the injured spinal cord were not altered after injury and suggests that FAIR protocols optimized to measure SCBF provide more value in the context of acute traumatic injury to the cord. T1-relaxation time constants were strongly related to injury severity and had a larger extent of changes than either SCBF or DWI measures. These findings suggest that perfusion decreases in the spinal cord can be monitored non-invasively after injury, and multi-parametric MRI assessments of perfusion, diffusion, and relaxation capture unique features of the pathophysiology of preclinical injury.
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