Assessment of gliosis around moveable implants in the brain.
Assessment of gliosis around moveable implants in the brain.
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DOI：
10.1088/1741-2560/6/4/046004
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发表时间：
2009-08
影响因子：
4
通讯作者：
Muthuswamy J
中科院分区：
文献类型：
--
作者：
Stice P;Muthuswamy J
植入后重新定位微电极是一种有前途的方法，可以实现单个神经元记录的长期可靠性。本研究的主要目的是(a)评估植入后脑内微电极运动的神经胶质反应，(b)确定植入后脑内微电极运动导致神经胶质反应最小的最佳时间窗口。11只Sprague-Dawley大鼠植入了两个微电极，每个微电极在植入后可以在体内移动。研究了三个队列：(1)微电极在第2天移动（n = 4只动物），(2)微电极在第14天移动（n = 5只动物），(3)微电极在第28天移动（n = 2只动物）。各组1-3在运动后4周（分别为植入后30天、42天和56天）进行组织学评估。此外，5只对照动物植入了不移动的微电极。对照动物分别于(1)30天（n = 1）、(2)42天（n = 2）和(3)56天（n = 2）植入后进行组织学评估。定量评估微电极尖端周围的胶质纤维酸性蛋白（GFAP）表明，与30天的对照组相比，第2天移动微电极周围的GFAP水平相似。然而，在第14天和第28天移动的微电极尖端周围的GFAP表达水平显著低于植入42天和56天的对照微电极周围的表达水平。因此，我们得出结论，植入后移动微电极是一种可行的策略，不会对脑组织造成任何额外的损伤。此外，在植入14天后，将微电极向下移动实际上可能会长期降低微电极尖端周围GFAP的表达水平。
Repositioning microelectrodes post-implantation is emerging as a promising approach to achieve long-term reliability in single neuronal recordings. The main goal of this study was to (a) assess glial reaction in response to movement of microelectrodes in the brain post-implantation and (b) determine an optimal window of time post-implantation when movement of microelectrodes within the brain would result in minimal glial reaction. Eleven Sprague-Dawley rats were implanted with two microelectrodes each that could be moved in vivo post-implantation. Three cohorts were investigated: (1) microelectrode moved at day 2 (n = 4 animals), (2) microelectrode moved at day 14 (n = 5 animals) and (3) microelectrode moved at day 28 (n = 2 animals). Histological evaluation was performed in cohorts 1–3 at four-week post-movement (30 days, 42 days and 56 days post-implantation, respectively). In addition, five control animals were implanted with microelectrodes that were not moved. Control animals were implanted for (1) 30 days (n = 1), (2) 42 days (n = 2) and (3) 56 days (n = 2) prior to histological evaluation. Quantitative assessment of glial fibrillary acidic protein (GFAP) around the tip of the microelectrodes demonstrated that GFAP levels were similar around microelectrodes moved at day 2 when compared to the 30-day controls. However, GFAP expression levels around microelectrode tips that moved at day 14 and day 28 were significantly less than those around control microelectrodes implanted for 42 and 56 days, respectively. Therefore, we conclude that moving microelectrodes after implantation is a viable strategy that does not result in any additional damage to brain tissue. Further, moving the microelectrode downwards after 14 days of implantation may actually reduce the levels of GFAP expression around the tips of the microelectrodes in the long term.
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影响因子：
4.6
作者：
Muthuswamy, J;Okandan, M;Jain, T
通讯作者：
Jain, T
影响因子：
4.6
作者：
Franks, W;Schenker, I;Hierlemann, A
通讯作者：
Hierlemann, A
影响因子：
3
作者：
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影响因子：
3
作者：
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通讯作者：
Heinemann, Uwe
影响因子：
4.6
作者：
Muthuswamy, J;Okandan, M;Gilletti, A
通讯作者：
Gilletti, A