Role of Thyroid Hormone and Nerve Growth Factor in the Development of Choline Acetyltransferase and Other Cell‐Specific Marker Enzymes in the Basal Forebrain of the Rat
Role of Thyroid Hormone and Nerve Growth Factor in the Development of Choline Acetyltransferase and Other Cell‐Specific Marker Enzymes in the Basal Forebrain of the Rat
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甲状腺激素和神经生长因子在大鼠基底前脑胆碱乙酰转移酶和其他细胞特异性标记酶发育中的作用
DOI：
10.1111/j.1471-4159.1988.tb02984.x
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发表时间：
1988
影响因子：
4.7
通讯作者：
A. Hunt
中科院分区：
文献类型：
--
作者：
A. J. Patel;M. Hayashi;A. Hunt
研究分别和联合使用L-甲状腺素（从出生即第1天起每天皮下注射0.3μg/g体重）和2.5S神经生长因子（NGF；出生后第1、3、5、7和9天脑室内注射2μg）治疗对10日龄大鼠基底前脑中不同细胞类型的生化发育的影响。分别根据胆碱乙酰转移酶（ChAT）、谷氨酸脱羧酶（GAD）和谷氨酰胺酶活性监测胆碱能、γ-氨基丁酸能（GABAergic）和谷氨酸能神经元的发育，而使用谷氨酰胺合成酶（GS）和2',3'-环状核苷酸-3'-磷酸水解酶（CNPase）活性判断星形胶质细胞和少突胶质细胞的成熟程度。从出生开始接受甲状腺激素或 NGF 治疗可显着增加新生大鼠基底前脑 ChAT 活性的表达。当两种药物给予同一只动物时，ChAT 活性的刺激远大于个体效应的总和，这与我们早期的体外研究结果一致。在甲状腺功能减退大鼠中，虽然 NGF 增强了甲状腺素诱导的 ChAT 活性的增加，但低剂量使用的 NGF 的显着作用并未检测到。在目前的实验条件下，甲状腺素和NGF处理对谷氨酰胺酶、GS和乳酸脱氢酶的活性均没有明显影响。然而，给予甲状腺素显着增加了正常大鼠的 CNPase 活性，而在甲状腺功能减退大鼠中，对 CNPase 和 GAD 的影响也很显着。 NGF 处理后未观察到 CNPase 和 GAD 活性的类似升高，这表明 NGF 的作用是针对胆碱能细胞的。这些观察结果进一步支持了我们的观点，即皮质下胆碱能神经元受到甲状腺激素和局部体液因子（如 NGF）之间相互作用的调节。
The effects of treatment with l‐thyroxine (subcu‐taneously 0.3 μg/g body weight daily from birth, i.e., day 1) and 2.5S nerve growth factor (NGF; intraventricularly 2 μg on 1, 3, 5, 7, and 9 postnatal days), separately and together, were studied on the biochemical development of different cell types in the basal forebrain of 10‐day‐old rats. The development of cholinergic, γ‐aminobutyric acid‐ergic (GABAergic), and glutamatergic neurons was monitored respectively in terms of choline acetyltransferase (ChAT), glutamate decarboxylase (GAD), and glutaminase activities, whereas glutamine synthetase (GS) and 2′,3′‐cyclic nu‐cleotide‐3′‐phosphohydrolase (CNPase) activities were used to judge the maturation of astroglial and oligodendroglial cells. Treatment with either thyroid hormone or NGF from birth significantly increased the expression of ChAT activity in the basal forebrain of neonatal rats. When both agents were administered to the same animal, in agreement with our earlier in vitro findings, the stimulation in ChAT activity was much greater than the sum of the individual effects. In hypothyroid rats, significant effects of NGF at the low doses used were not detectable, although the increase of ChAT activity induced by thyroxine was potentiated by NGF in these animals. Under the present experimental conditions neither thyroxine nor NGF treatment had an appreciable effect on the activities of glutaminase, GS, and lactate dehydrogenase. However, the administration of thyroxine markedly increased CNPase activity in normal rats, whereas in hypothyroid rats the effect on both CNPase and GAD was also significant. Similar elevations in CNPase and GAD activities were not observed after NGF treatment, suggesting that the effect of NGF was specific to the cholinergic cells. The observations provide further support for our proposal that subcortical cholinergic neurons are subject to regulation by an interaction between thyroid hormone and local humoural factors such as NGF.
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新的神经元生长因子。
影响因子：
13.9
作者：
Berg,DK
通讯作者：
Berg,DK
DOI：
10.1073/pnas.81.8.2562
发表时间：
1984-04
影响因子：
11.1
作者：
E. Recio-Pinto;F. Lang;D. Ishii
通讯作者：
E. Recio-Pinto;F. Lang;D. Ishii
DOI：
10.1016/0169-328x(86)90020-3
发表时间：
1986-07-01
期刊：
MOLECULAR BRAIN RESEARCH
影响因子：
--
作者：
MOBLEY, WC;RUTKOWSKI, JL;JOHNSTON, MV
通讯作者：
JOHNSTON, MV
影响因子：
56.9
作者：
T. Large;S. Bodary;D. Clegg;G. Weskamp;U. Otten;L. Reichardt
通讯作者：
T. Large;S. Bodary;D. Clegg;G. Weskamp;U. Otten;L. Reichardt
DOI：
10.1073/pnas.78.8.4733
发表时间：
1981-01-01
期刊：
PROCEEDINGS OF THE NATIONAL ACADEMY OF SCIENCES OF THE UNITED STATES OF AMERICA-BIOLOGICAL SCIENCES
影响因子：
--
作者：
SEELIG, S;LIAW, C;OPPENHEIMER, JH
通讯作者：
OPPENHEIMER, JH