Use of gel electrophoresis and Raman spectroscopy to characterize the effect of the electronic structure of single-walled carbon nanotubes on cellular uptake.
Use of gel electrophoresis and Raman spectroscopy to characterize the effect of the electronic structure of single-walled carbon nanotubes on cellular uptake.
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DOI：
10.1021/ac403827m
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发表时间：
2014-03-18
影响因子：
7.4
通讯作者：
Pantano, Paul
中科院分区：
文献类型：
--
作者：
Chilek, Jennifer L.;Wang, Ruhung;Draper, Rockford K.;Pantano, Paul
众所周知，活细胞对单壁碳纳米管的摄取取决于单壁碳纳米管的长度和表面化学等因素。令人惊讶的是，关于单壁碳纳米管的电子结构是否会影响吸收，人们知之甚少。其中一个原因是缺乏衡量细胞群体对单壁碳纳米管摄取的方法。在此之前，我们开发了一种快速、灵敏且无标记的十二烷基硫酸钠(SDS)-聚丙烯酰胺凝胶电泳法(PAGE)来测量从培养细胞制备的裂解产物中单壁碳纳米管的数量(Wang等人)。、、)。在这里，我们描述了使用SDS-PAGE和微探针拉曼光谱来检测和区分哺乳动物细胞内化的单壁碳纳米管的电子结构。利用正常大鼠肾(NRK)细胞和牛血清白蛋白(BSA)分散的SWNTs，我们证明该方法可以在内化BSA-SWNTs的细胞裂解液中检测到金属和半导体SWNTs，并且NRK细胞对BSA-SWNTs的摄取不受SWNT电子结构的影响。
It is well-known that the uptake of single-walled carbon nanotubes (SWNTs) by living cells depends on factors such as SWNT length and surface chemistry. Surprisingly, little is known about whether the electronic structure of a SWNT influences uptake. One reason for this has been the lack of methods to measure the uptake of SWNTs by cell populations. Previously, we developed a rapid, sensitive, and label-free sodium dodecyl sulfate (SDS)-polyacrylamide gel electrophoresis (PAGE) method for measuring the amount of SWNTs in lysates prepared from cultured cells (Wang et al. , , ). Herein, we describe the use of SDS-PAGE and microprobe Raman spectroscopy to detect and distinguish the electronic structure of SWNTs internalized by mammalian cells. Using normal rat kidney (NRK) cells and SWNTs dispersed with bovine serum albumin (BSA), we demonstrate that the method can detect both metallic and semiconducting SWNTs in lysates of cells that had internalized BSA-SWNTs and that the uptake of BSA-SWNTs by NRK cells is not influenced by SWNT electronic structure.
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