Expression of developmental myosin and morphological characteristics in adult rat skeletal muscle following exercise-induced injury
Expression of developmental myosin and morphological characteristics in adult rat skeletal muscle following exercise-induced injury
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运动损伤后成年大鼠骨骼肌发育肌球蛋白的表达及形态特征
DOI：
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发表时间：
1999
期刊：
影响因子：
--
通讯作者：
N. McKee
中科院分区：
文献类型：
--
作者：
H. K. Smith;M. Plyley;C. Rodgers;N. McKee
摘要本研究测定了离心偏置运动损伤后成年大鼠骨骼肌肌纤维中肌球蛋白重链（MHCd）发育亚型表达的程度和稳定性，及其与细胞形态的关系。成年雌性Wistar大鼠[274（10）g]被指定为非运动对照组或进行30分钟的跑步机运动（等级，−16°;速度，15 m · min−1），然后在恢复后1、2、4、7或12天处死（每组n = 5-6）。采用光学显微镜和数字成像技术对比目鱼肌（S）、股中间肌（VI）和胫骨前肌（TA）进行组织学和免疫组织学染色的连续横向冷冻切片检查。在TA中很少检测到MHCd阳性（MHCd+）的纤维。在VI和S组中，与所有其他组合并（0.2%，1.2%; P ≤ 0.01）相比，在运动后4天和7天，在大鼠中观察到更高比例的MHCd+纤维（分别为0.8%和2.5%）。在S中，运动后12天（0.7%）观察到的MHCd+纤维频率低于运动后7天（2.6%）。大多数（85.1%）的MHCd+纤维的形态特征指示的损伤，变性，修复或再生。大多数的MHCd+纤维也表达成人慢，和/或快肌球蛋白重链。在数量上，MHCd+纤维比不表达MHCd的纤维更小（<2500 μm2）且更有角。因此，有一个短暂的增加，在一个小的，但不同的人口MHCd+纤维后不习惯，功能锻炼成年大鼠的S和VI肌肉。观察到的密切耦合的MHCd表达与肌纤维内的形态学变化表明，这些特征有一个共同的，初始运动诱导的损伤相关的刺激。
Abstract The extent and stability of the expression of developmental isoforms of myosin heavy chain (MHCd), and their association with cellular morphology, were determined in adult rat skeletal muscle fibres following injury induced by eccentrically-biased exercise. Adult female Wistar rats [274 (10) g] were either assigned as non-exercised controls or subjected to 30 min of treadmill exercise (grade, −16°; speed, 15 m · min−1), and then sacrificed following 1, 2, 4, 7, or 12 days of recovery (n = 5–6 per group). Histologically and immunohistologically stained serial, transverse cryosections of the soleus (S), vastus intermedius (VI), and tibialis anterior (TA) muscles were examined using light microscopy and digital imaging. Fibres staining positively for MHCd (MHCd+) were seldom detected in the TA. In the VI and S, higher proportions of MHCd+ fibres (0.8% and 2.5%, respectively) were observed in rats at 4 and 7 days post-exercise, in comparison to all other groups combined (0.2%, 1.2%; P ≤ 0.01). In S, MHCd+ fibres were observed less frequently by 12 days (0.7%) than at 7 days (2.6%) following exercise. The majority (85.1%) of the MHCd+ fibres had morphological characteristics indicative of either damage, degeneration, repair or regeneration. Most of the MHCd+ fibres also expressed adult slow, and/or fast myosin heavy chain. Quantitatively, the MHCd+ fibres were smaller (<2500 μm2) and more angular than fibres not expressing MHCd. Thus, there was a transient increase in a small, but distinct population of MHCd+ fibres following unaccustomed, functional exercise in adult rat S and VI muscles. The observed close coupling of MHCd expression with morphological changes within muscle fibres suggests that these characteristics have a common, initial exercise-induced injury-related stimulus.
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DOI：
10.1242/jeb.176.1.135
发表时间：
1993
期刊：
The Journal of experimental biology
影响因子：
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作者：
Armstrong,RB;Taylor,CR
通讯作者：
Taylor,CR
影响因子：
3.3
作者：
STPIERRE, BA;TIDBALL, JG
通讯作者：
TIDBALL, JG
影响因子：
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作者：
ARMSTRONG, RB;PHELPS, RO
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PHELPS, RO
功能超载和运动的大鼠跖肌纤维中 MHC 同种型的调节。
DOI：
10.1152/jappl.1997.83.1.280
发表时间：
1997
期刊：
Journal of applied physiology (Bethesda, Md. : 1985)
影响因子：
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作者：
Roy,RR;Talmadge,RJ;Fox,K;Lee,M;Ishihara,A;Edgerton,VR
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DOI：
10.1152/ajplung.1990.259.2.l116
发表时间：
1990
期刊：
The American journal of physiology
影响因子：
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作者：
LaFramboise,WA;Daood,MJ;Guthrie,RD;Butler-Browne,GS;Whalen,RG;Ontell,M
通讯作者：
Ontell,M