Pannexin 3 is required for late stage bone growth but not for initiation of ossification in avian embryos.
Pannexin 3 is required for late stage bone growth but not for initiation of ossification in avian embryos.
复制标题
DOI：
10.1002/dvdy.24425
复制
发表时间：
2016-09
期刊：
影响因子：
--
通讯作者：
Richman JM
中科院分区：
文献类型：
--
作者：
Bond SR;Abramyan J;Fu K;Naus CC;Richman JM
Pannexin 3 （PANX3）是一种通道形成蛋白，能够在体外刺激成骨。本研究利用RCAS逆转录病毒系统研究了PANX3在鸡胚软骨内骨形成过程中的过表达和低表达。在肢体中，PANX3 RNA首先在软骨凝聚中检测到，并局限于骨骺、骨干和软骨膜的增厚前软骨。PANX3的增加并不足以改变成骨，但是含有干扰RNA结构的病毒导致骨体积减少20%。含有shEGFP磁带的对照病毒不影响发育。有趣的是，表型局限于晚期，而不是成骨间质的增殖，软骨凝结的形成或肥厚带的形成。此外，使用QPCR或放射性原位杂交，Hh、WNT、FGF或BMP信号的读数也没有变化。根据PANX3的正常表达域和相对较晚的表型表现，可能需要PANX3半通道来促进肥厚软骨细胞向成骨细胞的转变，从而实现最终的骨大小。
Pannexin 3 (PANX3) is a channel-forming protein capable of stimulating osteogenesis in vitro. Here we studied the in vivo roles of PANX3 in the chicken embryo using the RCAS retroviral system to over-express and knockdown expression during endochondral bone formation. In the limbs, PANX3 RNA was first detected in the cartilage condensations and became restricted to the prehypertrophic cartilage of the epiphyses, diaphysis and perichondrium. The increase in PANX3 was not sufficient to alter osteogenesis, however a virus containing an interference RNA construct caused a 20% reduction in bone volume. The control virus containing a shEGFP cassette did not affect development. Interestingly, the phenotype was restricted to later stages rather than to proliferation of the skeletogenic mesenchyme, formation of the cartilage condensation or creation of the hypertrophic zones. In addition, there was also no change in readouts of Hh, WNT, FGF or BMP signaling using either QPCR or radioactive in situ hybridization. Based on the normal expression domains of PANX3 and the relatively late manifestation of the phenotype, it is possible that PANX3 hemichannels may be required to facilitate the transition of hypertrophic chondrocytes to osteoblasts, thereby achieving final bone size.
登录
查看更多内容
影响因子：
3.5
作者：
Chau M;Forcinito P;Andrade AC;Hegde A;Ahn S;Lui JC;Baron J;Nilsson O
通讯作者：
Nilsson O
影响因子：
4.8
作者：
Hsu, Shu-Hsuan C.;Zhang, Xiaoyun;Alman, Benjamin A.
通讯作者：
Alman, Benjamin A.
影响因子：
6.2
作者：
Bond, Stephen R.;Lau, Alice;Naus, Christian C.
通讯作者：
Naus, Christian C.
影响因子：
4.1
作者：
Berendsen AD;Olsen BR
通讯作者：
Olsen BR
RCAS-RNAI：一种发育中的鸡视网膜的功能丧失方法。
影响因子：
--
作者：
Harpavat, S;Cepko, CL
通讯作者：
Cepko, CL